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Immune colloidal gold reagent for detecting orchid virus series and its preparation

A colloidal gold test paper, colloidal gold technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of inability to detect on-site, high detection cost, long detection time, etc., and achieve simple operation, fast detection speed, and production low cost effect

Inactive Publication Date: 2008-10-29
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a kind of immune colloidal gold reagent suitable for orchid virus CyMV and ORSV and preparation method thereof, to solve the disadvantages of the prior art such as long detection time, inability to detect on-site, high detection cost, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: utilize CyMV immune colloidal gold test strip to detect the method for orchid CyMV virus, comprising the following steps:

[0039]1) Collection and processing of samples to be tested: Weigh 0.5 grams of sample leaves and put them in a mortar, pour the sample buffer solution into the mortar for grinding, after grinding, take the sample solution and centrifuge at 10,000rpm for 5 minutes, and take the supernatant, which is ready to be tested The sample solution is ready for use.

[0040] 2) Detection:

[0041] a. Return the CyMV immune colloidal gold test strip to room temperature before testing;

[0042] b. Add 3-5 drops of the sample solution to be tested in the polyclonal antibody display area of ​​the CyMV immunocolloidal gold reagent strip;

[0043] c. After 5 minutes to 30 minutes of reaction, judge the result.

[0044] 3) Interpretation of results:

[0045] If there is no red band in the CyMV polyclonal antibody display area of ​​the CyMV immune co...

Embodiment 2

[0046] Embodiment 2: Utilize ORSV immune colloidal gold test strip to detect the method for orchid ORSV virus

[0047] Utilize the method of ORSV immune colloidal gold test strip to detect orchid ORSV virus: change the CyMV immune colloidal gold test strip in embodiment 1 into ORSV immune colloidal gold test strip, and all the other detection methods are the same as the detection method in embodiment 1.

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PUM

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Abstract

The invention discloses an immune colloidal gold test strip used for detecting cymbidium virus series and preparing method thereof, comprising an immune colloidal gold test strip used for detecting cymbidium CyMV virus and ORSV virus and preparing method thereof; the immune colloidal gold test strip used for detecting cymbidium virus series has the advantages of accurate result, fast determination, simple and convenient operation and low cost, etc., does not need washing process and standard contrast, can detect the samples in batches or singly, and is easy for promotion and use; the operator does not need professional training and can complete the operation according to the specification.

Description

technical field [0001] The invention relates to a reagent for detecting Cymbium mosaic virus (CyMV) and Odontoglossum ringspotvirus (Odontoglossum ringspotvirus, ORSV) by using an immunocolloidal gold method and a preparation method thereof. Background technique [0002] Orchid is a famous flower in the world. There are 30,000 to 35,000 orchid native species, 800 genera, and 60,000 to 80,000 varieties of orchids in the world. Many orchids have high horticultural and ornamental value, and are known as "green stocks". Some provinces and cities have begun to cultivate orchids on a large scale for export and earn foreign exchange. Taiwan of my country also grows a variety of orchids, and its technology and scale are at the international leading level. [0003] However, in the production process, orchids are often harmed by viral diseases, which can cause symptoms such as leaf spots, necrosis, and flower discoloration and deformity of orchid plants, seriously affecting their qu...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/544G01N33/532G01N33/52
Inventor 吴祖建林志铿谢联辉谢荔岩罗金水
Owner FUJIAN AGRI & FORESTRY UNIV
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