Immune colloidal gold reagent for detecting orchid virus series and its preparation
A colloidal gold test paper, colloidal gold technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of inability to detect on-site, high detection cost, long detection time, etc., and achieve simple operation, fast detection speed, and production low cost effect
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Embodiment 1
[0038] Embodiment 1: utilize CyMV immune colloidal gold test strip to detect the method for orchid CyMV virus, comprising the following steps:
[0039]1) Collection and processing of samples to be tested: Weigh 0.5 grams of sample leaves and put them in a mortar, pour the sample buffer solution into the mortar for grinding, after grinding, take the sample solution and centrifuge at 10,000rpm for 5 minutes, and take the supernatant, which is ready to be tested The sample solution is ready for use.
[0040] 2) Detection:
[0041] a. Return the CyMV immune colloidal gold test strip to room temperature before testing;
[0042] b. Add 3-5 drops of the sample solution to be tested in the polyclonal antibody display area of the CyMV immunocolloidal gold reagent strip;
[0043] c. After 5 minutes to 30 minutes of reaction, judge the result.
[0044] 3) Interpretation of results:
[0045] If there is no red band in the CyMV polyclonal antibody display area of the CyMV immune co...
Embodiment 2
[0046] Embodiment 2: Utilize ORSV immune colloidal gold test strip to detect the method for orchid ORSV virus
[0047] Utilize the method of ORSV immune colloidal gold test strip to detect orchid ORSV virus: change the CyMV immune colloidal gold test strip in embodiment 1 into ORSV immune colloidal gold test strip, and all the other detection methods are the same as the detection method in embodiment 1.
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