Method for testing content of nosiheptide preparation

A detection method, nosiheptide technology, applied in the field of biochemistry, can solve the problems of repeatability of test results, poor fat solubility of diluents, and poor quality

Inactive Publication Date: 2008-11-05
ZHEJIANG ESIGMA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is aimed at the poor fat-soluble diluent used in the existing Nosiheptide preparation content determination method (antibiotic microbiological test method), the situation that the larger deviation of test results and poor repeatability can be produced in the detection process, and provide An improved detection method ensures the accuracy of determination

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  • Method for testing content of nosiheptide preparation

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Experimental program
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Embodiment Construction

[0022] 1. 8 batches of 1% nosiheptide premix were tested and compared by the original method and the method of the present invention respectively, and the results are shown in Table 1 below.

[0023] Table 1: 1% nosiheptide premix sample biological potency determination results and related deviation statistics:

[0024] Sample number

Measured value without organic solvent (%)

Measured value with organic solvent (%)

1#(DMF)

0.7620

1.0060

2#(DMF)

0.7860

1.0180

3#(DMF)

0.8260

0.9880

4# (chloroform)

0.8620

0.9960

5# (ethanol)

0.9620

1.0080

6# (methanol)

0.9120

0.9960

7# (acetone)

0.8920

1.0100

8# (dimethyl sulfoxide)

0.9960

1.0040

average

0.8748

1.0033

standard deviation

0.0821

0.0095

RSD

9.3800

0.9467

[0025] 2. 8 batches of Nosiheptide purified prod...

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Abstract

The present invention provides a method for detecting the content of Nosiheptide preparation. The improvement of the method is that an organic solvent is added beforehand to protect the stability before diluting the Nosiheptide preparation with an oil-insoluble system or ebb-oil-soluble system. The organic solvent which is used for adding beforehand is the organic solvent series which has a certain liposolubility to Nosiheptide, such as DMF, dimethyl sulfoxide, acetone, chloroform, methanol, ethyl and the like. Preferably the organic solvent is DMF. The invention uses adding organic solvent beforehand to protect the stability of the Nosiheptide in dissolving-out and after-treatment to make that the Nosiheptide can be quantitatively and stably transferred to the oil-insoluble dilution thereby the content can be accurately measured or used for the dilution dispersion process with other purpose. The method of the invention evidently improves the accuracy, precision and repeatability of normal detecting method.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and relates to an improved method for detecting the content of a feed drug additive and various preparations thereof, in particular to a protection method for the sulfur-containing polypeptide antibiotic nosiheptide in the dilution process. Background technique [0002] Feed safety is the basic point of green and environmentally friendly food. my country has a vast territory and a large population. With the acceleration of reform and opening up and the improvement of people's living standards, the demand for livestock, poultry and aquatic products is also increasing. In addition, my country is a large agricultural country, and the export volume of poultry and livestock products is very large, but the problem of drug residues is very serious. Therefore, the research and development of green feed additives is very necessary for the protection of people's health and the development of my country...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38
Inventor 丁瑜曹春华陈贵才
Owner ZHEJIANG ESIGMA BIOTECH CO LTD
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