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Fish family establishing and disease-resistant high yield fine breeding method

An improved breeding method and a technology for establishing a method, which can be applied in the field of disease-resistant and high-yield breeding of improved varieties, and can solve the decline; Chen Wei et al. (2005) carried out molecular research on the screening of flounder microsatellite markers and genetic diversity; Zhang Yuxi (2006, research work (Wang Xincheng et al., 1997; Wang Xiaoqing et al., 2006; Su Pengzhi et al., 2008), and also carried out some molecular marker screening and genetic diversity evaluation, germplasm identification and disease resistance-related molecular marker screening, etc. For example, Zou Shuming et al. (2000) used RAPD technology to detect and analyze the genetic structure of aquaculture populations of flounder and turbot; You Feng et al. Analysis of genetic diversity and other issues to achieve the effect of fast growth and strong disease resistance

Active Publication Date: 2011-01-26
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

In terms of marine fish genetics and breeding research, artificial gynogenetic breeding of parthenogenetic seedlings has been mainly carried out on flounder, large yellow croaker and turbot (Wang Xincheng et al., 1997; Wang Xiaoqing et al., 2006; Su Pengzhi et al., 2008 ), and at the same time, some research work on molecular marker screening and genetic diversity evaluation, germplasm identification and molecular marker screening related to disease resistance has also been carried out, such as Zou Shuming et al. Detection and analysis of population genetic structure; You Feng et al. (2001) used isozyme technology to analyze the genetic diversity of flounder and cultured flounder in coastal Shandong; Liu et al. (2005a, b) used microsatellite markers and AFLP technology Evaluation of the genetic structure of the cultured and wild populations of flounder showed that the genetic diversity of the cultured population has declined to a certain extent; Chen Wei et al. (2005) carried out microsatellite marker screening and molecular research on genetic diversity of flounder; Zhang Yuxi et al (2006) analyzed the MHC gene polymorphisms of flounder disease-resistant fish and non-disease-resistant fish, and preliminarily screened the MHC genotypes related to disease resistance; Not yet reported

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Embodiment Construction

[0041] Take the establishment of disease-resistant populations and families of flounder and the selection of disease-resistant high-yield fine varieties as an example below, in conjunction with the accompanying drawings, the technical content of the present invention is described in detail:

[0042] The technical content of the present invention is as follows: including the technical method of establishment of flounder family and breeding of disease-resistant and high-yielding fine varieties;

[0043] 1. A method for establishing a flounder family; including: (1) preparation of a disease-resistant colony; (2) establishment of a family; (3) marking methods for different families.

[0044] (1) Preparation of disease-resistant populations (RS) of flounder: natural selection and artificial infection were used to obtain disease-resistant populations (RS).

[0045] Natural selection: During the peak season of flounder disease in the summer of 2003-2004, go to the flounder farms in L...

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Abstract

The invention relates to a method for the fish genealogy establishment and the disease resistance and high yield improved type selection. By adopting the technologies, such as group selection, genealogy selection, hybridization and artificial infection, as well as the sieving of the quick growth genealogy and disease resistance genealogy, the genealogy which has the advantages of strong flounder disease resistance and quick growth is selected, and the selection method for the disease resistance and high yield high quality improved type fish is established. The technology has the advantages ofstrong pertinence, high selection efficiency, obvious effect, etc. and the survival percent of the disease resistance flounder fish fry is more than 80 percent, which is increased by 30 percent more than the survival percent of the common flounder fish fry; the growth speed of the disease resistance flounder fish fry is also increased by more than 10 percent. The method in the invention opens up a new technical approach for culturing the disease resistance fish variety. As a disease resistance high yielding variety selection method applied to the improved fish type selection, the method is suitable for the popularization and the application on all the cultured fishes, and has important significance and application value in culturing the cultured fish of disease resistance and high yield improved type.

Description

Technical field: [0001] The invention belongs to a method for breeding disease-resistant and high-yield fish species in the field of aquaculture, and is a method for breeding disease-resistant and high-yield improved species that can be applied in the breeding of improved fish species. Background technique: [0002] Fish is an important aquaculture species and an important food source for humans. my country is the country with the highest aquaculture production in the world. At present, the annual output of farmed fish in my country has reached more than 20 million tons. In addition to satisfying the domestic market, about 300,000 tons of fish products are exported abroad each year, which is one of the main agricultural products for my country's export earnings. However, in recent years, with the rapid development of the fish farming industry, frequent diseases, germplasm degradation, and low survival rate of seedlings have caused economic losses of more than 15 billion yua...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00C12N1/20
CPCY02A40/81
Inventor 陈松林田永胜徐田军邓寒沙珍霞刘本伟季相山于过才
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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