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Method for detecting porphyra yezoensis polysaccharides PY-D2 anti-tumour and enhancing immunity

A technology for enhancing immunity and laver polysaccharides, applied in the preparation of test samples, biochemical equipment and methods, biological testing, etc.

Inactive Publication Date: 2008-11-19
SHANGHAI OCEAN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

At present, there have been no reports on the anti-tumor and immunity-enhancing effects of Porphyra zebra polysaccharide PY-D2. The immune system also plays an important regulatory role and has the effect of stimulating lymphocyte proliferation. Lymphocyte proliferation response is a commonly used and very important immune index. Therefore, on the one hand, it can be explained that the Porphyra zebra polysaccharide PY-D2 plays a role in improving the body's immunity. On the other hand, the enhancement of the body's immunity can reduce the incidence of cancer and indirectly inhibit the growth of tumor cells, which indicates that PY-D2 polysaccharide PY-D2 has a broad application prospect in tumor treatment

Method used

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  • Method for detecting porphyra yezoensis polysaccharides PY-D2 anti-tumour and enhancing immunity
  • Method for detecting porphyra yezoensis polysaccharides PY-D2 anti-tumour and enhancing immunity
  • Method for detecting porphyra yezoensis polysaccharides PY-D2 anti-tumour and enhancing immunity

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Embodiment Construction

[0035] The method for detecting the anti-tumor and immunity-enhancing effects of the polysaccharide PY-D2 of the present invention will be further described in detail below.

[0036] The specific implementation is as follows:

[0037] 1. Polysaccharide extraction and purification

[0038] 1) After thawing, Porphyra variegata fronds were dried and shredded, refluxed with alcohol for 3 hours to degrease, and dried naturally in the shade. After drying in the shade, the samples were added with water at a ratio of 1:50 to extract polysaccharides, heated at a constant temperature of 100°C for 3 hours, suction filtered, collected and extracted After the extract was concentrated, it was centrifuged at 4000rpm for 10min to discard the sediment, and the Sevag method was used for deproteinization. The supernatant was added with 95% ethanol, and the precipitate was obtained after centrifugation at 4000rpm for 10min, and the crude product was obtained after lyophilization. polysaccharide;...

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Abstract

Disclosed is a method for detecting effects of Porphyra yezoensis polysaccharide PY-D2 on tumor resistance and immunity enhancement. The method for detecting effects of Porphyra yezoensis polysaccharide PY-D2 on tumor resistance and immunity enhancement is mainly characterized in that the detection method adopts the following steps of: polysaccharide extraction and purification; detecting the tumor cell growth by MTT assay; analyzing the cell cycle of tumor cells with a flow cytometer; data processing; preparation and primary culture of mouse spleen lymphocyte; detecting the survival rate of mouse spleen lymphocyte by MTT assay; detecting the lymphocytes cycle with the flow cytometer; and data processing. The method for detecting effects of Porphyra yezoensis polysaccharide PY-D2 on tumorresistance and immunity enhancement can reveal a significant role of the Porphyra yezoensis polysaccharide PY-D2 played in the treatment of tumors, meanwhile, the method also effectively promotes theapplication value of the Porphyra yezoensis polysaccharide PY-D2 in the treatment of tumors.

Description

Technical field: [0001] The invention relates to a method for detecting the anti-tumor and immunity-enhancing effects of the Porphyra variegata polysaccharide PY-D2. Background technique: [0002] Studies have shown that marine plant polysaccharides, as anti-tumor active substances, are not only rich in resources, but also have low toxic and side effects. For example, they can overcome the damage to normal cells in the body while killing tumor cells during chemotherapy and radiotherapy for cancer patients. , looking for highly effective and low-toxic anticancer drugs from marine plant polysaccharides is one of the important contents of current tumor research. [0003] Porphyra variegata is purplish red with a slight blue tinge, and belongs to the family Rhodophyta Rubiaceae in classification. It is a high-protein, low-fat and nutritious edible seaweed. At present, there have been no reports on the anti-tumor and immunity-enhancing effects of Porphyra zebra polysaccharide PY...

Claims

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Application Information

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IPC IPC(8): G01N33/48G01N1/34C12Q1/02
Inventor 何培民张陆曦
Owner SHANGHAI OCEAN UNIV
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