Calcitonin-gene-related peptide and trout calcitonin amalgamation polypeptide

A salmon calcitonin and fusion peptide technology, applied in the direction of peptides, hybrid peptides, specific peptides, etc., can solve the problems of calcitonin gene-related peptides staying in the laboratory research stage

Inactive Publication Date: 2008-12-10
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Currently there are only calcitonin products on the market, and calcitonin gene-related peptide is only in the laboratory research stage

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0011] Specific embodiment 1: The amino acid sequence of calcitonin gene-related peptide and salmon calcitonin fusion polypeptide in this embodiment is as follows:

[0012] AlaCysAspThrAlaThrCysValThrHisArgLeuAlaGlyLeuLeuSerArgSerGlyGlyValValLysAsnAsnPheValProThrAsnValGlySerLysAlaPhe GlyThr CysSerAsnLeuSerThrCysGlyLeuGlyLysLeuSerGlnGluAlaHisLysLeuGlnThrProArgThrAsnThrGlySerGlyThrPro; and calcitonin gene-related peptide and salmon calcitonin fusion polypeptide C-terminal amidation.

[0013] In this embodiment, the fusion gene of calcitonin gene-related peptide and salmon calcitonin was synthesized by Nanjing Jitian Co., Ltd., and the fusion polypeptide was obtained from strains constructed in our laboratory, which were transformed into tomatoes and purified by affinity chromatography.

[0014] In this embodiment, the calcitonin gene-related peptide is connected to a coding sequence called an "affinity handle" in the salmon calcitonin fusion gene, and this "affinity handle" can ...

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PUM

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Abstract

The invention provides calcitonin gene-related peptide and salmon calcitonin fusion polypeptide, relating to fusion polypeptide. In order to generate a product containing both calcitonin and the calcitonin gene-related peptide, the invention provides the calcitonin gene-related peptide and the salmon calcitonin fusion polypeptide. The amino acid sequence of the calcitonin gene-related peptide and the salmon calcitonin fusion polypeptide is shown in SEQ ID No:1; moreover, C-terminal amidation is carried out to the calcitonin gene-related peptide and the salmon calcitonin fusion polypeptide. Experiments verify that the calcitonin gene-related peptide and the salmon calcitonin fusion polypeptide preserve respective activities of the calcitonin gene-related peptide and salmon calcitonin.

Description

technical field [0001] The present invention relates to a fusion polypeptide. Background technique [0002] Calcitonin (CT) can directly inhibit the proliferation of osteoclasts (OC), prevent osteolysis, promote bone formation, and can quickly relieve the pain or onset of lumbar cone disease caused by osteoporosis. One of the drugs of choice for osteoporosis. It can also effectively regulate the balance between bone calcium and blood calcium, and has an analgesic effect; it is effective for the treatment of hypercalcemia, osteitis deformans, hyperthyroidism, and bone pain caused by various reasons (such as rheumatoid, tumor, etc.) ) and so on have very obvious effects. In the treatment of bone pain, the analgesic effect of calcitonin can reach 30 to 50 times that of morphine. Because calcitonin can inhibit the secretion of gastric acid, it can be used to treat acute gastric ulcer; because calcitonin can inhibit the secretion of pancreatic hormones and inhibit the release o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00
Inventor 余琼
Owner HEILONGJIANG UNIV
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