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Production of glucosinolates from agricultural by-products and waste

A technology of glucosinolates and mixtures, applied in the field of glucoraphanin, can solve the problems of insufficient recognition of the health potential of glucosinolates, high costs, etc.

Inactive Publication Date: 2008-12-24
INTERCONTINENTAL GREAT BRANDS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enormous health potential of glucosinolates has not been fully appreciated due to the high cost of glucoraphanin sources

Method used

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  • Production of glucosinolates from agricultural by-products and waste
  • Production of glucosinolates from agricultural by-products and waste
  • Production of glucosinolates from agricultural by-products and waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Example 1 : Glucosinolates from cauliflower seeds

[0045] 15 g of cauliflower seeds were degreased by n-hexane extraction, and ground into powder and passed through a No. 18 sieve. The pulverized and defatted cauliflower seeds were added to 1500 ml of deionized water to form a mixture. The mixture was boiled for 5 minutes to inactivate β-thioglucosidase in the seeds. The total volume of the mixture was brought to 4500 ml by adding deionized water. One square foot of Electropure Excellion Anion Exchange Membrane Heterogeneous Strong Basic, Type 1, Model 1-200 (Snowpure, LLC in San Clemente, CA), was suspended in the continuously stirred mixture at 5°C for 24 hours. After extraction, the anion-exchange membrane was rinsed briefly with deionized water, and the membrane was placed in a stirred tank containing 4500 ml of 1N potassium chloride solution at 5°C for 4 hours to release glucosinolates from the anion-exchange membrane. Glucoraphanin concentrations in aqueous media we...

Embodiment 2

Example 2 : Glucosinolates from cauliflower florets

[0048] With the cauliflower floret that 150g pulverizes drying, repeat the process of embodiment 1. The cauliflower florets were ground to a powder to pass through a No. 18 sieve. The result of this process is that greater than 80% of the glucosinolates are recovered.

Embodiment 3

Example 3 : Plant-derived glucosinolates

[0049] The procedure of Example 1 was repeated by using 1 kg of freshly harvested cauliflower leaves and stems. Cauliflower leaves and stems were steamed for 20 minutes to reduce β-thioglucosidase activity. The cauliflower leaves and stems are then mashed. This process glucosinolate extraction result is similar with embodiment 1.

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Abstract

The present invention relates to a process for producing glucosinolates, particularly glucoraphanin, from cruciferous plants. More particularly, a method is provided for producing glucosinolates from agricultural by-products and waste. The general method comprises providing a mixture of glucosinolate-containing plant material and liquid, heating the mixture to inactivate enzymes in the plant material, contacting the heat inactivated mixture with an anion exchange membrane whereby at least a portion of the glucosinolates are extracted from the liquid and absorbed onto the anion exchange membrane, and releasing the glucosinolates from the anion exchange membrane. Preferably, the extraction and release steps are repeated at least once. The glucosinolates produce by the method of the invention may be incorporated into a variety of food products, pharmaceuticals, and health supplements.

Description

technical field [0001] The present invention relates to a method for the production of glucosinolates, in particular glucorphanin, from agricultural by-products and waste. Glucosinolates are chemoprotive precursor compounds. The glucosinolates produced by the method of the present invention can be incorporated into various foods, medicines, health supplements, and related products. Background technique [0002] It is generally believed that diet plays an important role in controlling the progression of cancer, and eating more fruits and vegetables can reduce the incidence of human cancer. Some trace chemical constituents present in plants provide major protective mechanisms when delivered into mammalian cells. In addition, drugs, nutritional supplements, or foods that are fortified or supplemented with cancer-fighting chemicals from plants may provide additional health benefits. An important trend in the food industry in the United States is the promotion of health-consci...

Claims

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Application Information

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IPC IPC(8): C07H15/18A61K31/7028A61K36/31A23L1/30A61P35/00A61P39/02A23L19/00A23L29/00
CPCA61P35/00A61P39/02C07H1/08C07H15/14
Inventor L·G·维斯特T·波默洛N·V·马图舍斯基C·J·路德维希J·赫斯特金C·克劳利N·-C·金
Owner INTERCONTINENTAL GREAT BRANDS
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