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Bovine serum cholesterol standard substance and use thereof

A standard substance, cholesterol technology, applied in the field of serum cholesterol standard substances, can solve the problems of high price, difficult to obtain blood source, poor safety, etc.

Active Publication Date: 2009-01-14
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, human serum cholesterol standard substances have problems such as difficulty in obtaining blood sources, poor safety, and high prices.

Method used

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  • Bovine serum cholesterol standard substance and use thereof
  • Bovine serum cholesterol standard substance and use thereof
  • Bovine serum cholesterol standard substance and use thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0057] Experimental Example 1 Uniformity Test

[0058] 1.1 Sampling method

[0059] After the bovine serum standard substances prepared according to the above examples were numbered sequentially, a random number table was used to determine the number of samples to be drawn, and 30 samples were randomly selected for uniformity testing.

[0060] 1.2 Measurement method

[0061] The enzymatic determination of cholesterol is simple, has sufficient sensitivity, and good precision (total variation coefficient is less than 1%, which is similar to the cholesterol determination method), so the enzymatic determination is selected as the method for detecting the homogeneity of bovine serum standard substance. The sample volume for the determination was 3 μL.

[0062] The Zhongsheng Cholesterol Kit (enzymatic method) was selected, and the OLYMPUS AU400 automatic biochemical analyzer was used to perform the measurement according to the instructions. The measurement was repeated 3 times fo...

experiment example 2

[0073] Experimental Example 2 Stability Monitoring Experiment

[0074] After monitoring the stability at a temperature of -18°C ± 2°C, it is determined by HPLC-internal standard method, x T is the measured value of each time period, X 0 is the initial measured value, and the rate of change is calculated according to the formula, and the results are shown in Table 3.

[0075] Table 3 Stability results of bovine serum standard substance at -18°C ± 2°C

[0076]

[0077] The average absolute change rate was 0.07%, which indicated that the bovine serum cholesterol standard substance had good stability.

experiment example 3

[0078] Experimental Example 3 Fixed value experiment

[0079] High performance liquid chromatography (HPLC)-internal standard method was used to determine the value and evaluate the uncertainty. High-performance liquid chromatography (HPLC)-internal standard method is recommended by the Chinese Medical Association as a reference method for cholesterol determination in my country, and the value is accurate and reliable. The amount is 205.0mg / dL±3.6mg / dL.

[0080] 3.1 Determination method - high performance liquid chromatography

[0081] Determination of bovine serum standard substance by high performance liquid chromatography (HPLC) cholesterol (Chen Wenxiang, Li Peiying, Wang Shu, etc. Recommended method for blood lipid determination of Chinese Medical Association Laboratory Society: reference method for determination of serum total cholesterol (draft). Chinese Medicine Inspection Journal, 1995, 18(2): 114~117), calibrated with the national first-class serum cholesterol stand...

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Abstract

The invention discloses a standard serum cholesterol substance and the application thereof. The standard serum cholesterol substance is prepared by a method with the following steps: (a) pellucid bovine serums are chosen and classified according to initial values that are measured by adopting a cholesterase method; (b) a high-concentration cholesterol concentrated solution is prepared by adopting an ultracentrifugation method, and an initial value is measured by adopting the cholesterase method; (c) a preparation concentration is preset, and a preparation proportion is computed for the preparation according to a required preparation content and the bovine serum classification; (d) an antiseptic agent is added; and (e) the product is filtered and separately packaged, wherein, sodium azide is taken as the antiseptic agent, with the usage ratio of 0.1 percent according to the weight and volume ratio, and the filtering carries out the aseptic filtering by using an asbestos filtering plate of 0.2 micrometer. The result of a series of experiments that are implemented by adopting the standard bovine serum cholesterol substance obtained by the invention indicates that the standard substance has small matrix effect, universality in partial systems, uniformity and good stability.

Description

technical field [0001] The invention belongs to the field of detection, and more specifically, the invention discloses a standard substance of serum cholesterol. Background technique [0002] Determination of serum cholesterol is an important indicator of nutrition research. Improving the accuracy of serum cholesterol determination depends on the establishment and implementation of a standardized analysis system. In the 1980s, the United States launched the National Cholesterol Education Program (NCEP). Internationally, especially in the United States, cholesterol is The most complete reference system, the earliest standardization work, and the most effective clinical test indicators. In my country, the Biochemical Laboratory of the Beijing Institute of Gerontology, Ministry of Health, has conducted standardized research on cholesterol determination for more than ten years, and established a relatively complete cholesterol reference system. [0003] At present, there is cho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92C12Q1/60
Inventor 赵宁刘海燕
Owner BIOSINO BIO TECH & SCI
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