Seedling exercising method of tissue culture seedling of Dendrobium officinale

Abstract

The invention relates to a hardening method of tissue cultured seedlings of Dendrobium candidum, which comprises the following steps: (a) selection of bottled tissue cultured seedlings: (b) management before taking out the seedlings from the bottle, comprising: sealing a bottle cap for hardening the seedling by increasingly strengthening illumination intensity; loosening bottle caps for hardeningthe seedlings and uncovering the bottle caps for hardening the seedlings; (c) treatment after taking out the seedlings from the bottles, comprising: washing the seedlings firstly. gently taking the seedlings out from the bottles, cleaning off the culture medium adhering to the seedlings in clean water, spreading out the seedlings to be thin, drying the seedlings in shade until the roots of the seedlings become white, and transplanting the seedlings after the roots are sterilized; (d) management after the transplantation, comprising: hardening the seedlings by increasingly raising illuminationintensity and gradually reducing moisture. By adopting the technical scheme, the hardened tissue cultured seedlings of endrobium candidum are robust, the leaf color is dark green, the root system is rich, and the survival rate of the transplant is greatly improved.

Description

technical field [0001] The invention relates to a seedling hardening method of tissue-cultured seedlings of Dendrobium candidum, belonging to the technical field of tissue-cultured seedlings of Dendrobium candidum. Background technique [0002] Dendrobium candidum, also known as Dendrobim Officinale Kimra et Migo, belongs to Angiosperm, Monocotyledon, Microorder, Orchidaceae, and Dendrobium. It is a precious Chinese medicinal material. Industrialized production is mainly through the cultivation of tissue culture seedlings for propagation. The tissue culture seedlings just produced from the tissue culture room have poor self-supporting ability, which is mainly manifested in the absorption function of roots, the dredging function of fleshy stems and the photosynthesis of leaves. Poor; the adaptability to the outside world is not strong, resulting in a low survival rate of transplanting. [0003] Seedling hardening is an important link before going from the tissue culture room...

AI Technical Summary

Problems solved by technology

[0004] However, according to the traditional seedling hardening method, the survival rate of dendrobium tissue culture seedlings is still unsatisfactory, which makes the cost of planting seedlings in factory production maintained at a relatively high level

Traditional seedling hardening methods have not fundamentally solved the problems of poor root absorption function, fragile plants and poor adaptability of dendrobium tissue culture seedlings

Therefore, traditional seedling hardening methods cannot guarantee the transplanting survival rate of Dendrobium tissue cultured seedlings, resulting in rotten roots and stems of tissue cultured seedlings and high mortality