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Plants having improved growth characteristics and a method for making the same

A plant and plant cell technology, applied in the field of molecular biology, can solve problems such as the researched NRT protein, unreported effects on seed yield, etc., to achieve the effect of improving growth characteristics

Inactive Publication Date: 2009-01-21
CROPDESIGN NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These plants showed increased nitrogen uptake efficiency, but no effect on seed yield was reported
Furthermore, this document also discloses that overexpression of nitrate transporters in plants does not confer favorable growth characteristics on these plants
Furthermore, NRT proteins have not been studied under normal growth conditions or for the complete plant life cycle

Method used

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  • Plants having improved growth characteristics and a method for making the same
  • Plants having improved growth characteristics and a method for making the same
  • Plants having improved growth characteristics and a method for making the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0277] Embodiment class I HDZip hox5 polypeptide and coding sequence

Embodiment 1

[0278] Example 1: Identification of SEQ ID NO: 1 and SEQ ID NO: 2 related sequences

[0279] Using database sequence search tools such as the Basic Local Alignment Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215:403-410; and Altschul et al. (1997) Nucleic Acids Res. 25:3389-3402), at Sequences (full-length cDNA, EST or genome) were identified in sequences maintained by the National Center for Biotechnology Information (NCBI) Entrez nucleotide database. Typically, the BLAST program is used to find regions of local similarity between sequences by comparing nucleic acid or polypeptide sequences to sequence databases and by calculating the statistical significance of the matches. For example, the TBLASTN algorithm is applied to the polypeptide encoded by the nucleic acid SEQ ID NO: 1, with default settings and a filter turned on to ignore sequences of low complexity. The output window of the analysis is a pairwise comparison and is ordered according to the probability scor...

Embodiment 2

[0286] Example 2: Alignment of Class I HDZip hox5 polypeptide sequences

[0287] AlignX from Vector NTI (Invitrogen), based on the popular progressive alignment Clustal algorithm (Thompson et al. (1997) Nucleic Acids Res 25: 4876-4882; Chenna et al. (2003) Nucleic Acids Res 31: 3497- 3500). The phylogenetic tree can be constructed using the proximity join clustering algorithm. The default values ​​are gap opening penalty 10, gap extension penalty 0, 1, and the weight matrix chosen is Blosum 62 (if peptides are aligned).

[0288] The results of the multiple sequence alignment are shown in figure 2 . The 3 main domains characterized from the N-terminal to the C-terminal orientation are heavily boxed and identified as the acidic box, class I homology domain, and six heptad-leucine zippers. The "conserved domain" encompasses these 3 domains. Additionally, the Trp tail and RPFF amino acid motifs are lightly boxed.

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Abstract

The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (referred to as YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (hereinafter referred to as YEP16); or having modulated expression of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.

Description

field of invention [0001] The present invention relates generally to the field of molecular biology and relates to methods for improving the growth characteristics of plants relative to corresponding wild-type plants or other control plants. More specifically, the present invention relates to methods for improving plant growth characteristics comprising modulating the class I homeodomain leucine zipper ( H omeo d omainleucine z ipper, HDZip) the expression of hox5 polypeptide or its homologue encoding nucleic acid; Or comprise regulating the expression of nitrate transporter (NRT) or its homologue encoding nucleic acid in plant; Y ield E nhancing P rotein 16, hereinafter referred to as YEP16) polypeptide or homologue encoding nucleic acid expression; or including regulating type I glycogen synthase kinase (I type shaggy-like kinase) or homologue encoding nucleic acid expression in plants. The present invention also relates to a plant with regulated class I HDZip hox5 poly...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00A01H5/10
CPCC12N9/12C12N15/82
Inventor A·I·桑兹莫林纳罗C·勒佐Y·海茨费尔德V·布多夫L·德维尔德D·因泽V·米隆诺弗
Owner CROPDESIGN NV
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