Rooting method of Dendrobium officinale shoot

Abstract

The invention discloses a rooting method for dendrobium officinale buds, comprising the following steps: 1) the rooting culture solution is poured into a culture vessel, and filter paper bridge or absorbent cotton is added to the culture vessel until the expanded filter paper bridge or the absorbent cotton is flush with the rooting culture solution; then the culture vessel is sealed and sterilized at high temperature; 2) a single dendrobium officinale bud with the height of 2.5 to 6.0cm is cut, and a dendrobium officinale bud with the height difference of 0 to plus or minus 0.5cm is inoculated in the same culture vessel to be cultured; 3) when at least 3 roots with the length of more than 2cm are grown on each bud in the culture vessel, the test tube plantlet can be obtained, and the culture is stopped. The rooting method for the dendrobium officinale bud of the invention can reduce the production cost and can improve the transplanting survival rate of the test tube plantlet.

Description

technical field [0001] The invention relates to a rooting method of dendrobium officinale buds. Background technique [0002] Utilizing plant tissue culture technology to rapidly propagate test-tube seedlings of Dendrobium candidum is a common method for large-scale production of Dendrobium candidum. At present, in the propagation system of Dendrobium candidum test-tube plantlets, the rooting medium often adopts solid medium, and agar or carrageenan is generally the most commonly used coagulant in solid medium. The application of solid medium not only increases the production cost (according to calculation, the cost of agar in the medium accounts for about 50%); in addition, in the rooting cycle of about 2 months, because it is a solid medium, the buds can only absorb local Nutrients, resulting in insufficient supply or waste of nutrients, and it is easy to damage the root seedlings during the steps of taking seedlings and cleaning the root agar, aggravating the invasion of...

AI Technical Summary

Problems solved by technology

The application of solid medium not only increases the production cost (according to calculation, the cost of agar in the medium accounts for about 50%); in addition, in the rooting cycle of about 2 months, because it is a solid medium, the buds can only absorb local Nutrients, resulting in insufficient supply or waste of nutrients, and it is easy to damage the root seedlings during the steps of taking seedlings and cleaning the root agar, aggravating the invasion of diseases and insect pests, and then reducing the survival rate of transplanting, resulting in increased production costs , thus seriously affecting and restricting the efficient and sustainable development of the industry