Use of decorin in preparing medicine for treating ischemic cerebrovascular disease

A decorin, cerebrovascular disease technology, applied in the field of biomedicine, can solve the problems that have not been reported in the literature on decorin ischemic cerebrovascular disease

Inactive Publication Date: 2009-02-11
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are no literature reports on the use of decorin in the treatment of ischemic cerebrovascular diseases

Method used

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  • Use of decorin in preparing medicine for treating ischemic cerebrovascular disease
  • Use of decorin in preparing medicine for treating ischemic cerebrovascular disease
  • Use of decorin in preparing medicine for treating ischemic cerebrovascular disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: decorin (decorin) is used for the in vivo animal experiment of treating ischemic cerebrovascular disease

[0017] The pharmacological experiment of making focal cerebral ischemia model by mouse suture method is a commonly used animal experiment to verify that drugs have the effect of preventing and treating ischemic cerebrovascular disease. Therefore, it is used to verify the effect of decorin on cerebral ischemia in experimental animals. Blood protection.

[0018] 1. Animals

[0019] ICR mice, 25-28g, male, 8 mice / cage for each group, divided into 3 groups. Bought from the Shanghai Experimental Animal Center of the Chinese Academy of Sciences (animal certificate number: Zhongke Hudongguanzi No. 99-003). Raised in a clean animal room.

[0020] 2. Drugs

[0021] According to different combinations, the experimental mice were divided into groups:

[0022] Sham operation group (A): no drugs were administered.

[0023] Operation group (B): given the same...

Embodiment 2

[0034] Embodiment 2: decorin (decorin) is used for the in vitro experiment of treating ischemic cerebrovascular disease

[0035] 1. Method:

[0036] Blank control group: Brain microvascular endothelial cells were cultured in the culture medium containing 10% fetal bovine serum DMEM LysoPC group: a) Add concentration of 10 to the medium containing 10% fetal bovine serum DMEM -7 LysoPC cultured brain microvascular endothelial cells.

[0037] b) adding a concentration of 10 to the medium containing 10% fetal bovine serum DMEM -6 LysoPC cultured brain microvascular endothelial cells.

[0038] c) adding a concentration of 10 to the medium containing 10% fetal bovine serum DMEM -5 LysoPC cultured brain microvascular endothelial cells.

[0039] Decorin group: Add concentration of 10 to the medium containing 10% fetal bovine serum DMEM -5LysoPC and decorin co-culture brain microvascular endothelial cells. After culturing for 24 hours, the cells were lysed to extract the protein....

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PUM

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Abstract

The invention relates to the field of medical technology. Decorin is a member of CSPG (chondroitin sulfate proteoglycan) family rich in leucine and sulfuric acid, and a large amount of researches in aspect of biological functions are concentrated on inhibiting the growth of tumor and preventing the fibrosis of such tissues as renal glomerulus, lung, liver and the like. The invention, through an experiment on integral animals of cerebral ischemia and an experiment on cells in vitro, is verified that the Decorin can reduce the thrombosis arisen from ischemia through reducing vascular permeability and inflammation reaction, thus playing a role for curing ischemic cerebrovascular disease. Therefore, the Decorin has good application prospect on the preparation of medicines for preventing and curing the ischemic cerebrovascular disease.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and relates to the application of decorin (DCN) in the preparation of drugs for treating ischemic cerebrovascular diseases. Background technique [0002] Decorin is a member of the leucine sulfate-rich chondroitin sulfate proteoglycan (CSPG) family, and its nucleoprotein is composed of 12 repeat sequences, which are composed of a leucine-rich 24 Composed of regions of amino acid residues, this nucleoprotein constitutes 80% of the protein portion. (Cabello-VerrugioC, Brandan E.A novel modulatory mechanism of transforming growth factor-β signaling through Decorin and LRP-1.J Biol Chem.2007;282:18842-18850) [0003] In fact, its protein sequence was reported as early as 1986 and human decorin proteoglycan was cloned (TKrusius and E Ruoslahti. Primary structure of an extracellular matrix proteoglycancore protein deduced from cloned cDNA. Proc Natl Acad Sci U S A. 1986 October; 83 (20): 7683-768...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P9/10
Inventor 杨鹏远芮耀诚卢玲宋旭辉熊青卉
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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