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Kit for early cancer metastasis hybridization in situ, detection method and application thereof

A detection kit and in situ hybridization technology, applied in the field of kits, can solve the problems of unreported detection technology, drug resistance of tumor cells, failure of anti-cancer war, etc., and achieve convenient operation, high sensitivity and strong specificity Effect

Inactive Publication Date: 2009-02-11
NATUREGEN BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]In 2005, the American Institute of Health, the Cancer Institute, the Centers for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer" , that is to say, the cancer mortality rate has not been reduced, and it lists several factors that lead to the failure of the anti-cancer war: 1. Tumor cell heterogeneity; 2. Tumor cell drug resistance; 3. Incomplete design of anticancer drugs, etc.
However, there is no report about the in situ hybridization detection kit and detection technology of UCP gene

Method used

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  • Kit for early cancer metastasis hybridization in situ, detection method and application thereof
  • Kit for early cancer metastasis hybridization in situ, detection method and application thereof
  • Kit for early cancer metastasis hybridization in situ, detection method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] An in situ hybridization detection kit for early metastasis of cancer, including hybridization probes, markers, and synergists, wherein the hybridization probe sequence is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0045] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0046] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0047] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0048] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0049] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0050] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0051] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0052] Chromogen A 175μl / tube 1 tube / box Yellow liquid

[005...

Embodiment 2

[0095] A kind of UCP gene in situ hybridization detection method and kit application

[0096] 1. Specimen processing

[0097] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0098] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0099] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0100] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with conditions c...

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Abstract

The invention relates to a kit for detecting in situ hybridization of early stage cancer metastases, comprising a hybridization probe and a label, and the sequence of the hybridization probe is shown in SEQ ID NO.1. The invention also provides a method for detecting in situ hybridization of UCP gene. In addition, the invention provides the application of the kit in the preparation of medicine for detecting early stage cancer metastases diseases, and the cancers includes liver cancer, lung cancer, gastric cancer, breast cancer, carcinoma of colon, prostatic cancer, uterine cancer, cancer of pancreas or carcinoma of urinary bladder. The kit provided by the invention has the characteristics of high sensitivity and better particularity. The detecting method of the invention is convenient and simple in operation, and can be generally used and popularized in the hospitals which are above county level.

Description

【Technical field】 [0001] The invention relates to a kit, in particular to an in situ hybridization detection kit for early metastasis of cancer and its detection method and application. 【Background technique】 [0002] According to the information provided by authoritative organizations at home and abroad, there are 1.7 million new cancer cases in my country every year, nearly 1.6 million deaths, and 6 million patients. In the world, there are 8 million new cancer patients every year, nearly 8 million deaths, and about 84 million patients. , by 2020 the number of people will double, which is a set of terrible figures. [0003] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors for the failure of the cancer war are: 1. Heterogeneity of tumor cell...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 裘建英张云福
Owner NATUREGEN BIOTECH SHANGHAI
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