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Application of Pestalotiopsis guepinii Stey. toxin in herbicide

A technology of Polychaeta pseudodiscoides and herbicides, applied in the direction of herbicides and algicides, applications, biocides, etc., can solve problems such as unseen

Inactive Publication Date: 2009-02-25
董晔欣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the search of relevant information, there is no relevant report on the formulation of herbicides of biological origin with the crude toxin of Trichosanthemidiscoides as the main component

Method used

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  • Application of Pestalotiopsis guepinii Stey. toxin in herbicide
  • Application of Pestalotiopsis guepinii Stey. toxin in herbicide
  • Application of Pestalotiopsis guepinii Stey. toxin in herbicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Inoculate the Polychaeta pseudodiscoides bacterial strain preserved by the inventor on a PDA plate, cultivate it under dark conditions at 25-30°C, and when the colony is about ф80mm, use a sterile puncher (ф5mm) to cut out the outer edge hyphae of the colony to prepare Mushroom cake. Put the PD medium in a 250mL Erlenmeyer flask, 150mL per bottle, quantitatively inoculate 3 pieces of bacteria cake, and culture at 150r / min at 25°C in the dark for 15 days. The mycelium is filtered out by double-layer absorbent cotton gauze from the culture solution of Trichosporum pseudodiscoides, and the crude toxin culture filtrate is obtained. Take 3000mL of the crude toxin culture filtrate, extract with 1500mL, 1000mL, and 500mL of ethyl acetate respectively, combine the extracted ethyl acetate, concentrate and dry it, and then dilute to 600mL with methanol to obtain the crude toxin. Add 100g of 1601 to 200mL of crude toxin, then add 700mL of water, and stir well to obtain 1000mL of ...

Embodiment 2

[0024] Inoculate the Polychaeta pseudodiscoides bacterial strain preserved by the inventor on a PDA plate, cultivate it under dark conditions at 25-30°C, and when the colony is about ф80mm, use a sterile puncher (ф5mm) to cut out the outer edge hyphae of the colony to prepare Mushroom cake. Put the corn flour culture medium in a 250mL Erlenmeyer flask, 150mL per bottle, quantitatively inoculate 3 pieces of bacteria cake, and culture at 150r / min at 25°C for 15 days in the dark. The mycelium is filtered out by double-layer absorbent cotton gauze from the culture solution of Trichosporum pseudodiscoides, and the crude toxin culture filtrate is obtained. Take 5000mL of the crude toxin culture filtrate, extract with 2500mL, 1500mL, and 1000mL of ethyl acetate respectively, combine the extracted ethyl acetate and concentrate it to near-dryness, then dilute to 1000mL with methanol to obtain the crude toxin. Add 120g of 1601 to 350mL of crude toxin, then add 530mL of water, and stir ...

Embodiment 3

[0029]Inoculate the Polychaeta pseudodiscoides bacterial strain preserved by the inventor on a PDA plate, cultivate it under dark conditions at 25-30°C, and when the colony is about ф80mm, use a sterile puncher (ф5mm) to cut out the outer edge hyphae of the colony to prepare Mushroom cake. Put the corn-soybean flour medium in 250mL Erlenmeyer flasks, 150mL per bottle, quantitatively inoculate 3 pieces of bacteria cakes, and culture them at 150r / min at 25°C in the dark for 15 days. The mycelium is filtered out by double-layer absorbent cotton gauze from the culture solution of Trichosporum pseudodiscoides, and the crude toxin culture filtrate is obtained. Take 4000mL of the crude toxin culture filtrate, extract with 2000mL, 1000mL, and 1000mL of ethyl acetate respectively, combine the extracted ethyl acetate and concentrate to dryness, then dilute to 800mL with methanol to obtain the crude toxin. Add 150g of 1601 to 500mL of crude toxin, then add 350mL of water, and stir well ...

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Abstract

The invention relates to the use of pestalotiopsis toxin, in particular to the application thereof in the field of weedicide. After being sprayed, the weedicide which is formed by the toxin and is diluted by water has preferable effect on controlling cockspur grass, crab grass, yard grass, wiregrass, speedwell, etc. The invention has the advantages that the new use of the pestalotiopsis toxin is created, and a new application field is developed; the crude pestalotiopsis toxin, which is applied in the weedicide, has obvious effect on weeding the grass, and has a favorable prospect in medical application.

Description

technical field [0001] The present invention relates to the application of the discidomycetin, especially the application in the field of herbicides. Background technique [0002] Pestalotiopsis guepinii (Desm) Stey. is a plant pathogenic fungus that can cause various diseases such as leaf spot of moso bamboo and crest of Chinese fir. The disease has a strong destructive effect on the host plant and can cause a large number of host plants to lose their leaves. This is well known to the public. In recent years, the separation and identification of herbicidal active substances from fungal metabolites, which can be directly used in weed control or simulate the structure of active substances, and artificially synthesize herbicides, has become a hot field in the research of weed control and the development of microbial herbicides. Through searching relevant information, there is no relevant report on the formulation of herbicides of biological origin with the crude toxin of P. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P13/00
Inventor 董晔欣张金林张剑
Owner 董晔欣
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