Heterocyclic amine compound analytical method in cigarette mainstream flue gas

A technology for mainstream cigarette smoke and analysis methods, which is applied in the direction of material analysis, material analysis by electromagnetic means, material separation, etc. Effect

Inactive Publication Date: 2009-03-25
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(Kataoka, H., Kijima, K.Maruo, G., Bull.Environ.Contam.Toxic.1998, 60:60.) There are shortcomings such as complex pretreatment and detection sensitivity in the above-mentioned methods, so the existing pretreatment is improved It is of great significance to establish an analytical method that is applicable to all hybrid amine compounds and is relatively simple, with good selectivity and high detection sensitivity.

Method used

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  • Heterocyclic amine compound analytical method in cigarette mainstream flue gas
  • Heterocyclic amine compound analytical method in cigarette mainstream flue gas
  • Heterocyclic amine compound analytical method in cigarette mainstream flue gas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The balanced and screened cigarettes were smoked according to the standard conditions stipulated in YC / T 29-1996, and the gaseous particulate matter of the mainstream smoke of 20 cigarettes was captured with a Cambridge filter.

[0021] Fold the Cambridge filter disc that traps the particulate matter into the Erlenmeyer flask, pipette 60 mL of 0.1 mol / L HCl / methanol (volume ratio 2:1) extract containing 60 ng internal standard (TriMeIQx), and ultrasonically extract at room temperature 30min, let stand for 5min. Take 45mL of the extract and load it on the MCX solid-phase extraction column (10ml of methanol and 10ml of water pre-activated), and then use 10ml of 0.1mol / L HCl, 15ml of methanol, and 15ml of methanol / water / ammonia solution (volume ratio 25:5:75) Wash and elute with a mixed solution of 30ml methanol / ammonia water (volume ratio 95:5), and carry out HPLC-MS / MS analysis after the eluate is concentrated.

[0022] HPLC-MS / MS analysis conditions: use C4 (YMC pack, ...

Embodiment 2

[0028] The Kentucky reference cigarette 3R4F was smoked according to the standard conditions stipulated in YC / T 29-1996, and the particulate matter of 20 cigarettes was collected by the Cambridge filter. The filter disc is placed in the Erlenmeyer flask, and a certain amount of Glu-p-2, Glu-p-1, IQ and PHIP mixed solution is added on the filter disc with a microsampling needle, and then carried out according to the steps described in Example 1 Sample cleanup analysis. Five parallel experiments were carried out, and the results showed that the relative standard deviation of each compound was between 1.62-6.76%, indicating that the method had good reproducibility and stability.

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Abstract

The invention relates to a method for analyzing heterocyclic amine compounds in mainstream cigarette smoke. The method is characterized in that the analytic method utilizes a cambridge filter to trap the heterocyclic amine compounds in the mainstream cigarette smoke; the heterocyclic amine compounds are extracted by a HCL/ methanol solution, are purified through an MCX ion exchange column, condensed and detected by liquid chromatography-tandem mass spectrometry (HPLC-MS/MS); and the contents of the main heterocyclic amine compounds in the mainstream cigarette smoke are mensurated. Compared with the prior method for analyzing the heterocyclic amine compounds in the mainstream cigarette smoke, the analytic mensuration method does not need a complicated sample treatment step, does not need to derive a sample, has high sensitivity, good repeatability and reclaiming rate, and is suitable for analyzing the samples with large batches.

Description

technical field [0001] The invention belongs to a new method for pretreatment and analysis of heterocyclic amine compounds in cigarette mainstream smoke. The method uses Cambridge filter to capture the main heterocyclic amine compounds in the particulate matter of mainstream smoke, uses MCX solid phase extraction column for purification pretreatment, and then uses liquid chromatography-tandem mass spectrometry for analysis. Background technique [0002] The impact of smoking on health has attracted worldwide attention, and it is the duty-bound responsibility of tobacco industry workers to reduce the release of harmful components in cigarette smoke. HOFFMANN published a list of 69 harmful ingredients in 2001, including 8 heterocyclic amine compounds. These compounds are produced by the pyrolysis of organic compounds such as proteins and amino acids, and are widely found in the environment and daily food, and they have strong mutagenic activity, and some have also been shown ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02G01N27/64G01N1/34
Inventor 赵阁王海艳王昇颜权平郭吉兆刘绍峰谢复炜
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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