Preparation method for prepared rehmannia root polysaccharide

Abstract

The invention relates to a preparation method of prepared rehmannia root polysaccharide, which effectively resolves the problem that the prepared rehmannia root polysaccharide is extracted from the prepared rehmannia root polysaccharide. The technical proposal for the resolution is that water with 8 times of bulkfactor is added into the prepared rehmannia root, leaching is carried out for 4 hours at the temperature of 98 DEG C to 100 DEG C, filtration is carried out, water with 8 times of bulkfactor of original drug is added in dregs, leaching is carried out for 4 hours at the temperature of 98 DEG C to 100 DEG C, water in the dregs is squeezed completely, filtration is carried out, filtrates are combined and concentrated to 0.5g / mL of raw drugs, alcohol is added and mixed to lead alcohol content to reach 80 percent, slow addition and fast mixture are carried out, depositing in alcohol is carried out for 24 hours, suction filtration is carried out, 95 percent alcohol with 4 times of bulkfactor of the drugs is used for abstersion and sedimentation and volatized till no alcohol odor is left, water is added to dissolve to 2 times of bulkfactor of the crude drugs, pH is adjusted to 8 to 9, anhydrous calcium chloride is added to lead W / V to reach 7 percent, dissolution is carried out, room temperature is applied for the placement thereof, centrifugation is carried out, supernatant is taken for concentration to thick paste, and the prepared rehmannia root polysaccharide is obtained after drying at reduced pressure of is carried out at the temperature of 95 DEG C. The anhydrous calcium chloride deprives the protein of the prepared rehmannia root polysaccharide and avoids the use of poisonous reagent, therefore, the protein deprivation efficiency reaches 50.54 percent and the preservation ratio of the prepared rehmannia root polysaccharide reaches 77.15 percent. Therefore, the preparation method has enormous economic and social benefits.

Description

1. Technical field [0001] The invention relates to medicine, in particular to a preparation method of Rehmannia glutinosa polysaccharide. 2. Background technology [0002] Rehmannia glutinosa is derived from the fresh or dried rhizome of Rehmanniag Lutinosa Libosch, a plant commonly used in traditional Chinese medicine. Rehmannia glutinosa is a processed product of rehmannia glutinosa, which has the functions of replenishing essence and marrow, nourishing yin and blood, and is used for liver and kidney yin deficiency, dizziness and tinnitus, waist weakness, bone steaming hot flashes, internal heat and thirst, blood deficiency and chlorosis, Palpitations, irregular menstruation, metrorrhagia, dizziness, tinnitus, premature graying of beard and hair, etc. Modern studies have shown that rehmannia glutinosa contains glycosides, sugars, amino acids and other components, and pharmacological experiments have shown that rehmannia glutinosa polysaccharides have anxiolytic activity. ...

AI Technical Summary

Problems solved by technology

There are also many studies on the pharmacological effects and content determination of Rehmannia glutinosa polysaccharides. Although there are many extraction methods, they are all laboratory researches and cannot be put into production.

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