Laccase inducer and use thereof for improving microbial laccase production ability

Abstract

The invention discloses a compound inducer for improving the capacity of a microorganism strain for producing a laccase, which comprises bagasse, ethanol and a bluestone liquid; and a comparison research carried out on a whiterot fungi strain XG8 by utilizing the compound inducer shows the laccase capacity in a fermentation liquid is improved by 10 times than that of the strain not added with thecompound inducer. The inducer of the invention is quite suitable for being applied to the large-scale production of industrialized laccases.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a laccase inducer and its application in improving the ability of microorganisms to produce laccase. Background technique

[0002] White rot fungi are the most important lignin-degrading bacteria in nature, which produce and secrete a series of enzymes that can degrade lignin and its xenobiotics, which mainly include lignin peroxidase, manganese peroxidase and laccase . Laccase is mainly secreted by basidiomycetes that cause white decay of wood. It is an important lignin-degrading enzyme and belongs to a class of polyphenol oxidases containing copper. It can oxidize phenolic compounds through molecular oxygen and remove phenolic compounds in wood and pulp. Lignin, which decolorizes and degrades effluents in the pulp and paper industry, can significantly reduce the discharge of toxic pollutants into the environment.

[0003] It has been reported that phenolic substrates,...

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