Laccase inducer and use thereof for improving microbial laccase production ability

A technology of inducers and microorganisms, which is applied in the biological field to achieve the effect of significant laccase induction effect and the effect of improving the ability of laccase production

Inactive Publication Date: 2009-04-15
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, bagasse, ethanol and CuSO have not been utilized in the prior art 4 A report on a laccase inducer synthesized from a solution

Method used

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  • Laccase inducer and use thereof for improving microbial laccase production ability
  • Laccase inducer and use thereof for improving microbial laccase production ability
  • Laccase inducer and use thereof for improving microbial laccase production ability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Strains

[0033] Edible fungus XG8 strain: purchased from Kunming Edible Fungi Research Institute, Kunming City, Yunnan Province.

[0034] 2. Media, inducers and culture conditions

[0035] 2.1 Medium

[0036] Medium composition: MgSO 4 ·7H 2 O, 0.5g, NaCl 0.5g, KH 2 PO 4 1.0g, NH 4 SO 4 1.0g, 1.0g yeast powder, 15g glucose, add water to 1 liter, adjust pH to 5-6. This medium is mainly used for preserving strains and as a basic medium in induction experiments.

[0037] 2.2 Inducers

[0038] CuSO 4 Solution: prepare 1g.L -1 CuSO 4 The original solution was sterilized at 115°C for 20 minutes. In the induction test, after the mycelium was cultured for four days, 1g.L of -1 CuSO 4 solution, so that CuSO in the culture medium 4 The final concentration is 5mg.L -1 , 8mg.L -1 , 10mg.L -1 , 20mg.L -1 , 50mg.L -1 .

[0039] Ethanol solution: Anhydrous ethanol is sterilized by filtering through a 0.2 μm filter membrane. In the induction test, after the...

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Abstract

The invention discloses a compound inducer for improving the capacity of a microorganism strain for producing a laccase, which comprises bagasse, ethanol and a bluestone liquid; and a comparison research carried out on a whiterot fungi strain XG8 by utilizing the compound inducer shows the laccase capacity in a fermentation liquid is improved by 10 times than that of the strain not added with thecompound inducer. The inducer of the invention is quite suitable for being applied to the large-scale production of industrialized laccases.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a laccase inducer and its application in improving the ability of microorganisms to produce laccase. Background technique [0002] White rot fungi are the most important lignin-degrading bacteria in nature, which produce and secrete a series of enzymes that can degrade lignin and its xenobiotics, which mainly include lignin peroxidase, manganese peroxidase and laccase . Laccase is mainly secreted by basidiomycetes that cause white decay of wood. It is an important lignin-degrading enzyme and belongs to a class of polyphenol oxidases containing copper. It can oxidize phenolic compounds through molecular oxygen and remove phenolic compounds in wood and pulp. Lignin, which decolorizes and degrades effluents in the pulp and paper industry, can significantly reduce the discharge of toxic pollutants into the environment. [0003] It has been reported that phenolic substrates,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12R1/645
Inventor 魏云林吴丽洁林连兵季秀玲井申荣
Owner KUNMING UNIV OF SCI & TECH
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