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Foreign fragment flanking sequence of transgenic rice line Kefeng No. 8 containing sck/cry1Ac bivalent insect-resistant gene and use

A technology of transgenic rice and flanking sequences, applied in the fields of application, genetic engineering, plant gene improvement, etc.

Inactive Publication Date: 2009-04-22
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the analysis of existing patents and documents, it is found that there are no articles or patent reports about the flanking sequence of the foreign insert of the transgenic rice line Kefeng 8

Method used

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  • Foreign fragment flanking sequence of transgenic rice line Kefeng No. 8 containing sck/cry1Ac bivalent insect-resistant gene and use
  • Foreign fragment flanking sequence of transgenic rice line Kefeng No. 8 containing sck/cry1Ac bivalent insect-resistant gene and use
  • Foreign fragment flanking sequence of transgenic rice line Kefeng No. 8 containing sck/cry1Ac bivalent insect-resistant gene and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Cloning of the Side Sequence of the Foreign Insertion Vector of the Transgenic Rice Line Kefeng 8

[0039] 1. Experimental materials

[0040] 1. Plant material

[0041] Transgenic rice: the genetically modified rice line Kefeng 8.

[0042] 2. Enzymes and reagents

[0043] Molecular biology reagents, such as dNTPs, Taq DNA polymerase, and DL2000Marker were purchased from Dalian Bao Biological Engineering Co., Ltd.

[0044] Other biochemical reagents are imported subpackages or domestic analytical pure. Primers were synthesized by Beijing Aoke Biotechnology Co., Ltd.

[0045] 3. Experimental equipment

[0046] PCR instrument: Eppendorf Mastercycle gradient (Eppendorf co.)

[0047] Nucleic acid electrophoresis instrument: DYY-III nucleic acid electrophoresis instrument (Beijing Liuyi Instrument Factory)

[0048] DNA sequencer (Applied Biosystem377 automatic fluorescent sequence analyzer)

[0049] DNA electrophoresis analysis system: Kodak EDAS 290 (Kodak co.)

[0...

Embodiment 2

[0077] Qualitative PCR detection method based on the flanking sequence of the exogenous insert in the transgenic rice line Kefeng 8

[0078] 1. Experimental materials

[0079] 1. Plant material

[0080] Transgenic rice: the genetically modified rice line Kefeng 8.

[0081] Conventional rice: Minghui 86.

[0082] 2. Enzymes and reagents

[0083] Molecular biology reagents, such as dNTPs, Taq DNA polymerase, and DL2000 Marker were purchased from Dalian Bao Biological Engineering Co., Ltd.

[0084] Other biochemical reagents are imported subpackages or domestic analytical pure. Primers were synthesized by Beijing Aoke Biotechnology Co., Ltd.

[0085] 3. Experimental equipment

[0086] PCR instrument: Eppendorf Mastercycle gradient (Eppendorf co.)

[0087] Nucleic acid electrophoresis instrument: DYY-III nucleic acid electrophoresis instrument (Beijing Liuyi Instrument Factory)

[0088] DNA electrophoresis analysis system: Kodak EDAS 290 (Kodak co.)

[0089] Other instrum...

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Abstract

The invention relates to a flanking sequence for a foreign inserting fragment of a transgenic rice line Kefeng No. 8 harboring sck / crylAc double insect resistance genes and application thereof, which belong to the field of biotechnology. A flanking sequence of a 5' end is shown in SEQ ID NO1, and a flanking sequence of a 3' end is shown in SEQ ID NO2. The flanking sequence is utilized to design a specific primer for the augmentation of target DNA, which can establish sensitive and specific qualitative and quantificational PCR detection of the line specificity of the rice line Kefeng No. 8.

Description

technical field [0001] The present invention relates to a gene sequence in the field of biotechnology. Specifically, it relates to the flanking sequence of an exogenous insertion fragment of a transgenic rice line Kefeng 8. Background technique [0002] Rice is one of the most important food crops in my country. With the extensive research and application of transgenic technology in rice, several transgenic rice strains have been released to the environment and applied for commercial planting. Strain-specific detection of transgenic plants is an important technical basis for the supervision and management of transgenic plants to ensure their healthy development. The flanking sequence of the exogenous insert in a transgenic plant is one of the most important molecular characteristics of a transgenic plant line. Therefore, the flanking sequence of the exogenous insert is an important technical data for establishing a specific detection method for a transgenic plant line. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/10C12Q1/68
Inventor 谢家建王奕海王锡锋彭于发
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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