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Pig bagged freezing semen preservation liquor and preservation method thereof

A technology of freezing semen and preservation solution, which is applied to the preservation of human or animal bodies, biochemical equipment and methods, microorganisms, etc., can solve the problems of short storage time and inability to store semen for a long time, reduce the deformity rate of sperm, and benefit The effect of preservation and avoiding secondary pollution

Inactive Publication Date: 2009-04-29
ZHENGZHOU COLLEGE OF ANIMAL HUSBANDRY ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This invention can improve the semen utilization rate of excellent breeding boars to a certain extent, but the storage time of semen is relatively short, generally only about 7 days, and cannot be stored for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of frozen semen preservation solution in pig bags

[0028] Preparation of liquid A: Weigh 10 grams of glucose, 1 gram of lactose, 8 grams of sodium glutamate, and 8 grams of polyvinylpyrrolidone, dissolve them in 100 ml of distilled water, and then add them in order to completely dissolve them, and then add 10 grams of gentamicin sulfate. 10,000 IU, filtered through a bacterial filter, then added vitamin B 12 0.02 grams, 0.02 grams of sodium coffee, 10 milliliters of egg yolk, mixed thoroughly to form A liquid.

[0029] Preparation of liquid B: take 50 ml of liquid A, add 2 ml of glycerin and 2 ml of N-N-dimethylformamide, and mix thoroughly to obtain liquid B.

[0030] The reagents used above are required to be analytically pure, the pH values ​​of liquid A and liquid B are both 6.5-7.5, the osmotic pressure is 300-500 mmol / L, and they have a certain buffering capacity for acids and alkalis.

[0031] (2) Utilize the method for preserving pig semen of ...

Embodiment 2

[0039] (1) The preparation of pig bagged frozen semen preservation solution is different from Example 1 in that:

[0040] Ingredients of liquid A: 1 gram of glucose, 1 gram of lactose, 1 gram of sodium glutamate, 1 gram of polyvinylpyrrolidone, 100 ml of distilled water, 200,000 international units of gentamicin sulfate, vitamin B 12 0.02 grams, 0.02 grams of sodium coffee, egg yolk 10 ml.

[0041] Ingredients of liquid B: 60 ml of liquid A, 2 ml of glycerin, 2 ml of N-N-dimethylformamide.

[0042] (2) Utilize the method for preserving pig semen of above-mentioned frozen semen preservation solution, comprise the following steps:

[0043] 1. Concentration of sperm collection and seminal fluid: with embodiment one.

[0044] 2. Cool down: Dilute isothermally at 25°C according to the ratio of 1:1 between liquid A and concentrated semen, and then cool down to 4°C in the refrigerator for 1 hour.

[0045] 3. Balance: Dilute the semen cooled to 4°C for the second time at 4°C at a rat...

Embodiment 3

[0048] (1) The preparation of the frozen semen preservation solution in pig bags is the same as that of Example 1, the difference is that:

[0049] Ingredients of liquid A: 3 grams of glucose, 10 grams of lactose, 5 grams of sodium glutamate, 3 grams of polyvinylpyrrolidone, 100 ml of distilled water, 150,000 international units of gentamicin sulfate, vitamin B 12 0.03 grams, 0.04 grams of sodium coffee, 30 ml of egg yolk.

[0050] Ingredients of liquid B: 75 ml of liquid A, 5 ml of glycerin, and 4 ml of N-N-dimethylformamide.

[0051] (2) The preservation method utilizing pig bagged frozen semen preservation solution comprises the following steps:

[0052] 1. Concentration of sperm collection and seminal fluid: with embodiment one.

[0053] 2. Cool down: Dilute isothermally at 20°C at a ratio of 1:1.5 between liquid A and concentrated semen, and then cool down to 1°C in the refrigerator for 2 hours.

[0054] 3. Equilibrium: Dilute the semen cooled to 1°C for the second tim...

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PUM

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Abstract

The invention discloses a liquid for preserving bagged frozen pig semen and a method for preserving the same. The preservation liquid consists of liquid A and liquid B, wherein the liquid A contains 1 to 10 grams of glucose, 1 to 10 grams of lactose, 1 to 8 grams of sodium glutamate, 1 to 8 grams of polyvinylpyrrolidone, 100 milliliters of distilled water, 100 to 200 thousand of international units of antibiotic, 0.02 to 0.1 gram of vitamin B12, 0.02 to 0.1 gram of caffeine sodium benzoate, and 10 to 30 milliliters of egg yolk; and the liquid B contains 50 to 100 milliliters of the liquid A, 2 to 10 milliliters of glycerol, and 2 to 10 milliliters of N-N-dimethylformamide. The method for preserving the semen comprises the following steps: concentrating the semen after sampling the sperm, and diluting and refrigerating the liquid A with the concentrated semen proportionally and isothermally; diluting the mixture by using the liquid B for the second time, and subpackaging the semen into bags; and preserving the bagged semen in liquid nitrogen. The method is effective in maintaining the vitality of the sperms, has simple and convenient use and low cost, and can meet the needs of artificial insemination of the frozen pig semen.

Description

1. Technical field: [0001] The invention relates to the field of animal reproduction, and relates to a semen preservation solution and a semen preservation method, in particular to a pig bag-packed frozen semen preservation solution and a semen preservation method. 2. Technical background: [0002] With the development of the pig industry, the artificial insemination of pig semen stored at room temperature can no longer meet the development of the modern pig industry, and the artificial insemination technology of pig frozen semen has become an urgent problem to be solved in pig production at home and abroad. [0003] Compared with storage of semen at room temperature, frozen semen of pigs can preserve good genes indefinitely (such as the protection of local pig breed resources), prolong the service life of boars, and not be affected by the death, injury, semen collection interval or breeding of boars. The limitation of sterility is conducive to the artificial use of high-per...

Claims

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Application Information

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IPC IPC(8): C12N5/06A01N1/02
Inventor 权凯张长兴魏红芳尹慧茹张松涛孟令坤黄炎坤高家登陈理盾
Owner ZHENGZHOU COLLEGE OF ANIMAL HUSBANDRY ENG
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