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Parasite egg detecting method

A detection method and parasite technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of inability to detect and poor accuracy, and achieve the effects of reliable results, wide application range and simple operation.

Inactive Publication Date: 2009-05-06
廖党金 +7
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of poor accuracy and inability to detect eggs of fasciola and acanthocephala in the detection of parasite eggs by traditional methods, the purpose of the present invention is to provide a fast, accurate, simple, and widely applicable parasitic Qualitative and quantitative detection method of insect eggs

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] Fasciola ova detection

[0021] 1. Quantitatively weigh 4.7 grams of feces from medium-sized animals or more than 27.5 grams of feces from large animals, and make a soup with water 3 times the volume of the quantitative feces.

[0022] 2. Filter with a copper sieve, and rinse with an appropriate amount of water while filtering.

[0023] 3. Precipitate the filtrate for 30 minutes; discard the supernatant, add water to precipitate, and repeat three times.

[0024] 4. Discard an appropriate amount of supernatant, and use a graduated cylinder to quantify the sediment (5 milliliters per gram of the checked medium-sized animal feces sediment, and 3 milliliters per gram of the checked large animal feces sediment).

[0025] 5. After fully shaking the sediment solution (it is enough to see that the fecal residue is uniform in the feces sediment solution with the naked eye), quickly move part of the sediment solution to the two counting chambers of the "counter" with a dropper. ...

Embodiment 2

[0030] Detection of disc fluke eggs before and after.

[0031] 1. Quantitatively weigh more than 2 grams of the feces of the inspected livestock, and make a soup with 3 times the volume of the quantitative feces.

[0032] 2. Filter with a copper sieve, and rinse with an appropriate amount of water while filtering.

[0033] 3. Precipitate the filtrate for 30 minutes; discard the supernatant, add water to precipitate, and repeat three times.

[0034] 4. Discard an appropriate amount of supernatant, and use a graduated cylinder to quantify the sediment (3 milliliters per gram of the checked medium-sized animal feces sediment, and 3 milliliters per gram of the checked large animal feces sediment).

[0035] 5. After fully shaking the sediment solution (it is enough to see that the fecal residue is uniform in the feces sediment solution with the naked eye), quickly move part of the sediment solution to the two counting chambers of the "counter" with a dropper.

[0036] 6. Observe ...

Embodiment 3

[0040] Detection of eggs of Acanthocephalus chinensis in swine

[0041] 1. Quantitatively weigh more than 2 grams of the feces of the inspected livestock, and make a soup with 3 times the volume of the quantitative feces.

[0042] 2. Filter with a copper sieve, and rinse with an appropriate amount of water while filtering.

[0043] 3. Precipitate the filtrate for 15 minutes; discard the supernatant, add water to precipitate, repeat three times.

[0044] 4. Discard an appropriate amount of supernatant, and quantify the precipitate with a graduated cylinder, that is, 5 ml of the precipitate per gram of the tested pig feces.

[0045] 5. After fully shaking the sediment solution (it is enough to see that the fecal residue is uniform in the feces sediment solution with the naked eye), quickly move part of the sediment solution to the two counting chambers of the "counter" with a dropper.

[0046] 6. Observe and count the parasite eggs in the square frames of the two counting cham...

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Abstract

The invention relates to a method for detecting parasitic eggs, which is carried out according to the following steps and is calculated according to the following formula: firstly, livestock feces to be detected are quantified, and water in the volume of 3 to 5 times of the volume of the quantified feces is added so that the feces and the water are stirred into paste; the paste is filtered by a copper screen while washed and filtered by a right amount of water; a filtrate is precipitated for 15 minutes to 60 minutes or is centrifugated, supernatant is discarded, water is added for precipitation, and the procedures are repeated for three times; a right amount of supernatant is discarded, and a measuring cylinder is used for quantifying precipitated liquid; after the precipitated liquid is shaken to be full uniform, a dropper is used for rapidly moving the precipitated liquid to two counter chambers of a counter; and finally, the parasitic eggs in square grids of the two counter chambers of the counter are observed and countered so as to calculate an EPG (Eggs per Gram) value according to the following formula: EPG = (the number of the eggs in the two small square grids of the counter * the volume of the precipitated liquid of the feces) / (the weight of the feces * 0.2). The invention has simple and rapid operation and reliable results, is suitable for detecting most of the parasitic eggs through the feces excretion eggs of people and livestock, and particularly, can qualitatively and quantificationally detect eggs hardly detected so far, such as the eggs of fasciola spp, acanthocephala and distomes, thereby having a wider range of application.

Description

technical field [0001] The invention relates to a rapid and accurate qualitative and quantitative detection technology of parasite eggs. Background technique [0002] At present, there are three main methods for the detection of parasite eggs: smear method, McBurney's method and Stuart's method. [0003] The smear method is to use tweezers or a small stick to take a little fresh feces, put it on a glass slide, add a few drops of clean normal water or 50% glycerin water, mix well, remove the coarse residue, add a cover glass, and put it under a microscope for inspection. The thickness of the tablet should be such that the handwriting under the tablet can be vaguely recognized through the liquid film of feces. This method is simple and easy, and is suitable for checking various coccidia oocysts, etc., but the detection rate is low and cannot be quantified. [0004] The Maxwell method utilizes the floating principle of eggs. First, two glass slides are used to make an egg cou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N33/48G01N1/28
Inventor 廖党金李江凌曹冶李红谢晶叶健强赵素君文豪
Owner 廖党金
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