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Phenanthrene pollutant soil repair method using biosurfactant and bacteriorhiza

A surfactant, a technology for polluted soil, applied in the field of polluted ecological restoration, can solve problems such as affecting the efficiency of soil restoration

Inactive Publication Date: 2009-06-17
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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Problems solved by technology

Although the promotion of mycorrhizal root development and root exudation, as well as the change of plant root morphology will increase the bioavailability of organic pollutants in the soil and the ability of plants to absorb organic matter to some extent, but the effect of soil on organic pollutants The locking effect will still limit the degradation and absorption of pollutants by microorganisms or plants, affecting the efficiency of soil remediation

Method used

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  • Phenanthrene pollutant soil repair method using biosurfactant and bacteriorhiza
  • Phenanthrene pollutant soil repair method using biosurfactant and bacteriorhiza
  • Phenanthrene pollutant soil repair method using biosurfactant and bacteriorhiza

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Embodiment Construction

[0028] In the following, the present invention will be described in further detail in combination with specific embodiments.

[0029] Weigh 1g of phenanthrene and treat it as 5mgkg -1 And the air-dried soil samples aged for two months were placed in centrifuge tubes, and surfactant solutions of different concentrations were added, fully shaken at room temperature for 48 hours, then centrifuged, and the supernatant was accurately drawn, and analyzed by high performance liquid chromatography. Determination. The results showed that the optimal concentrations of Triton X-100 and rhamnopyranosoctanoside were 0.5% and 0.05% respectively (see Figure 2 for the results).

[0030] According to the determined optimal surfactant concentration, desorption kinetic experiments at the optimal concentration were carried out. Weigh 1g of phenanthrene and treat it as 5mgkg -1 And the air-dried soil samples aged for two months were placed in centrifuge tubes, 0.5% and 0.05% Triton X-100 and rh...

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Abstract

The invention provides a method for restoring phenanthrene-contaminated soil by applying a biosurfactant and mycorhiza. The method mycorrhizals purple medic and adds the biosurfactant, and the purple medic and the biosurfactant act together to restore phenanthrene-contaminated soil and are compared with a chemical surfactant. The method comprises the following steps: inoculant is inoculated to a cultivated stroma, purple medic is mycorrhizaled, and the purple medic after pregermination is planted in the phenanthrene-contaminated soil, is added with biosurfactant of Rhanopylanoside and chemical surfactant of Triton X-100 which are compared with each other. The mycorhiza and the surfactant act together to remarkably reduce the residual concentration of phenanthrene in the body soil, and can remarkably improve the biological repair efficiency of the phenanthrene-contaminated soil. The restore efficiency of inoculating and Triton X-100 is less than that of inoculating Rhanopylanoside. The method has reference function on restoring other organic polluted soil.

Description

technical field [0001] The invention relates to a pollution ecological restoration technology, in particular to a phytoremediation method for phenanthrene-contaminated soil through the combined action of mycorrhizae and biosurfactants. Background technique [0002] PAHs compounds produced by incomplete combustion or pyrolysis of organic matter are typical organic hydrocarbon pollutants commonly seen in the environment, and are also representative toxic organic compounds ubiquitous in soil, with chronic toxicity and "three It has the effect of "causing" and is extremely harmful to the human body and the environment. Therefore, the removal of phenanthrene in soil and the remediation of polluted land have become an important research topic in the field of soil environment research. [0003] Phytoremediation is a way to repair soil by using the growth of plants to absorb, transform and transfer pollutants, and to promote the degradation and mineralization of pollutants in the s...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10A01C1/00A01G31/00C12N1/14C05G1/00
Inventor 张淑贞武耐英黄红林
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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