Laser speckle blood current imaging and analyzing method

Abstract

The invention discloses a laser speckle blood stream imaging analysis method. The method comprises: irradiating a laser beam on an object to be detected and continuously acquiring N frames of laser speckle images reflected by the object to be detected according to identical exposure time and frame intervals; taking pixels from a Ns*Ns spatial neighborhood corresponding to identical positions in each frame of image to form a pixel set; calculating a contrast Kst within the spatial neighborhood and then calculating the blood stream speed V(i, j) of a corresponding biological tissue by means of a relational expression of the contrast and the blood stream speed; and taking the blood stream speed value corresponding to each pixel as grey scale to establish a two-dimensional blood stream speed distribution map. The laser speckle blood stream imaging analysis method is based on space-time combined analysis of laser speckle blood stream imaging to carry out high time and spatial resolution imaging on blood stream of a biological issue, thereby realizing real-time, dynamic and high time and spatial resolution monitoring of two-dimensional blood stream distribution, blood vessel form and blood stream dynamic changes of the biological tissue. The analysis method is suitable for researching brain function imaging, neurophysiology, disease pathology and drug effect evaluation.

Description

technical field [0001] The invention belongs to the field of biological tissue blood flow imaging methods, in particular to a laser speckle blood flow imaging analysis method, which is especially suitable for studying local blood flow distribution and vascular morphology of biological tissues under physiological and pathological conditions with high temporal and spatial resolution. . Background technique [0002] Laser speckle blood flow imaging is widely used to study local two-dimensional blood flow changes in biological tissues under physiological and disease states. However, the existing analysis methods of laser speckle blood flow imaging cannot take into account the time resolution and spatial resolution of the image at the same time. Such as the patent document "Using Laser Speckle Imager to Monitor the Time-Space Response Characteristics of Mesenteric Microcirculation Blood Flow" (its publication number is CN1391869, and its publication date is 2002.07.12), and its ...

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Problems solved by technology

[0003] The purpose of the present invention is to provide a laser speckle blood flow imaging analysis method, which can solve the limitation that the existing laser speckle imaging method cannot take into account the spatial resolution and time resolution, and realize the two-dimensional analysis of blood flow in biological tissues. Real-time high-resolution imaging of distribution

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