Infectious bursal disease live-vaccine heat-proof freeze-dry protectant and preparation method thereof

Abstract

The invention discloses a heat resistant lyophilized protectant for infectious bursal disease living vaccine and a preparation method thereof. The heat resistant lyophilized protectant comprises the following compositions in percentage by mass volume: 2 to 7 percent of lactose, 0.2 to 0.6 percent of milk extract, 0.2 to 0.8 percent of lactoalbumin hydrolysate, and the balance being water for injection. The protectant and the method overcome the defects of complicated compositions, high cost and the like generally existing in the prior heat resistant lyophilized protectant; and the heat resistant lyophilized protectant has the advantages of good heat resistance, effective protection of the living vaccine, simple formulation compositions and formulation method, low production cost, mass production and the like, and can ensure that the infectious bursal disease living vaccine can be stored for more than 24 months at a temperature of between 2 and 8 DEG C, and for 1 month at 37 DEG C. The protectant and the method solve the problems of low-temperature refrigerating transportation, inconvenient storage, high cost and the like required by the infectious bursal disease living vaccine.

Description

technical field [0001] The invention relates to a protective agent for a live virus vaccine, in particular to a heat-resistant freeze-drying protective agent for a live chicken infectious bursal disease vaccine and a preparation method thereof, belonging to the field of live virus vaccine production for animals. Background technique [0002] Lyoprotectant and its preparation process are a key technology in the production of live vaccines, which have a great impact on the storage, transportation and use of vaccines. At present, in my country's veterinary vaccines, live freeze-dried vaccines account for a considerable proportion. The commonly used protective agents in China are mainly milk, sucrose, gelatin, etc., with simple formulations and poor protective functions. The freeze-dried vaccines of these traditional protective agents are required to be stored below -15°C, which causes great inconvenience to manufacturers and users, and limits the long-term storage and transport...

AI Technical Summary

Problems solved by technology

[0005] In the 1980s, developed countries began to conduct research on heat-resistant protective agents. At present, their freeze-dried live vaccines basically use heat-resistant protective agents, but there is no detailed report on the method.

However, the heat-resistant lyoprotectant has defects such as complex preparation methods and high production costs.

[0007] In short, the existing heat-resistant freeze-drying protective agents have problems such as complicated formula, inconvenient operation, and unsatisfactory protective performance.

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