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Lactobacillus micro-capsule as well as preparation method and use

A lactic acid bacteria microcapsule, lactic acid bacteria technology, applied in the directions of microcapsules, capsule delivery, application, etc., can solve the problems of large damage of lactic acid bacteria, poor gastric acid tolerance, and difficulty in dissolving the intestinal tract, and achieves high viable bacteria count of lactic acid bacteria and enhanced gastric acid resistance. Sexual, good enteric effect

Active Publication Date: 2011-09-21
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve: 1. in the microcapsule preparation process, the existing preparation technology is big to the injury of lactic acid bacteria, causes the number of live bacteria in the microcapsule to be low; Destruction of metal ions and other substances in the feed; poor gastric acid tolerance; difficult to dissolve in the intestinal tract, etc., provide a new type of lactic acid bacteria microcapsules with a large number of viable lactic acid bacteria, high activity, good stability, and strong enteric solubility

Method used

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  • Lactobacillus micro-capsule as well as preparation method and use
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  • Lactobacillus micro-capsule as well as preparation method and use

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Embodiment 1

[0031] The preparation of embodiment 1 lactic acid bacteria microcapsule

[0032] (1) Preparation of Lactobacillus acidophilus fermentation broth

[0033] ①Preparation of shake flask seed solution: inoculate Lactobacillus acidophilus preserved on the slant of the test tube into the liquid medium, and culture the shake flask at 36°C for 16 hours at a rotation speed of 150r / min to obtain the Lactobacillus acidophilus shake flask seed solution;

[0034] The liquid medium is a mixed solution prepared according to the following weight-to-volume ratio (W / V): beef extract 0.5%, yeast extract 0.5%, soybean peptone 1%, sodium chloride 0.5%, glucose 1%, calcium carbonate 0.2%, pH7.0~7.5, sterilized at 121°C for 15min, and set aside;

[0035] ②Preparation of first-grade seed solution of shake flask: inoculate the seed solution of Lactobacillus acidophilus into the first-grade seed medium with an inoculum amount of 10%, and cultivate it in a shake flask at 36°C for 8 hours at a speed of ...

Embodiment 2

[0041] The preparation of embodiment 2 lactic acid bacteria microcapsules

[0042] (1) preparation of lactobacillus acidophilus fermented liquid: preparation method is the same as embodiment 1;

[0043] (2) Preparation of core material: After mixing the Lactobacillus acidophilus fermentation liquid in step (1) with 50% skimmed milk powder, 0.5% glycerin, 0.5% sodium glutamate, 10% trehalose and 15% maltodextrin , pre-freeze at -80°C in an ultra-low temperature refrigerator for 1 hour, take it out and freeze-dry it in a vacuum freeze dryer. Pulverize and make bacterial powder as core material;

[0044] (3) coating: the core material is suspended in the fluidized bed, the wall material is sprayed and coated, sprayed into the mixed solution of 3% chitosan, 5% glycerin and 10% sucrose from one nozzle, and sprayed from another nozzle Spray 5% pectin, 10% gum arabic, 1% soybean protein isolate and 1% wheat protein mixture, the spray speed is 10ml / min and 15ml / min respectively, and...

Embodiment 3

[0045] The preparation of embodiment 3 lactic acid bacteria microcapsules

[0046] (1) preparation of lactobacillus acidophilus fermented liquid: preparation method is the same as embodiment 1;

[0047] (2) Preparation of core material: After mixing the Lactobacillus acidophilus fermentation liquid in step (1) with 15% skimmed milk powder, 3% glycerin, 5% sodium glutamate, 8% trehalose and 10% maltodextrin , pre-freeze at -80°C in an ultra-low temperature refrigerator for 1 hour, take it out and freeze-dry it in a vacuum freeze dryer. Pulverize and make bacterial powder as core material;

[0048] (3) coating: the core material is suspended in the fluidized bed, the wall material is sprayed and coated, sprayed into the mixed solution of 2% chitosan, 3% glycerin and 6% sucrose from one nozzle, Spray a mixture of 15% gum arabic, 1% carrageenan, 3% xanthan gum, 3% soy protein isolate and 1% zein at a spray speed of 8ml / min and 10ml / min, and coat the core material at the same tim...

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Abstract

The invention relates to a lactobacillus microcapsule, a method for preparing the same, and the use of the same. The lactobacillus microcapsule consists of an outer layer of wall material, a freeze dried protective agent and lactobacilli. The invention also provides the method for preparing the lactobacillus microcapsule and the use of the lactobacillus microcapsule as a feed additive. The lactobacillus microcapsule can effectively protect the lactobacilli in a core material, prolong the survival time of the lactobacilli at room temperature and improve the tolerance of the lactobacilli to themetal ions in the feed; in addition, the lactobacillus microcapsule has good gastric acid resistance and can disintegrate in the intestinal canal quickly to release the lactobacilli, thereby improving the utilization rate of the lactobacilli, balancing the micro-ecological environment in the intestinal canal, suppressing the growth of pathogenic bacteria, protecting the health of the intestinal canals of animals, reducing the incidence rate of the intestinal canals of the animals and the like.

Description

technical field [0001] The invention relates to a lactic acid bacteria microcapsule, which belongs to the technical field of microbial feed additives, in particular to a lactic acid bacteria microcapsule composed of an outer wall material, a freeze-drying protective agent and lactic acid bacteria, a preparation method and an application thereof. Background technique [0002] The wide application of antibiotics and other antibacterial drugs in animal husbandry has greatly promoted the development of animal husbandry. At present, more than 95% of premix products have added antibacterial drugs. But its disadvantages are also becoming more and more obvious: serious drug residues in animal husbandry products directly endanger human health; the abuse of antibiotics leads to drug resistance of pathogenic bacteria, resulting in the spread and spread of drug-resistant strains; at the same time, animal flora imbalance, animal immunity decline, the breeding environment deteriorates, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A23K10/18A23K40/30A61K35/744A61K35/747
Inventor 张玳华赵雁青莫云刘宇孙占敏贾秋英姚琨白玉卿赵军
Owner BEIJING DABEINONG TECH GRP CO LTD
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