Fast detection method and detection reagent kit for metronidazole, and its use in detection of cosmetic and disinfector added with metronidazole

A detection method, the technology of metronidazole, is applied in the direction of analyzing materials through chemical reactions, organic chemistry, and material analysis through observation of the influence of chemical indicators, which can solve the problems of expensive instruments, heavy detection workload, Large-scale on-site inspection and other issues to achieve the effect of simplifying the inspection steps, reducing inspection costs, and improving operational efficiency

Active Publication Date: 2009-08-12
广东省药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although the above-mentioned chromatography, ELISA method and dual-wavelength linear regression method, etc., can detect metronidazole in cosmetics or disinfectants, these methods have complex operations, long sample processing time, and cannot be used for large quantities of samples to be detected. To achieve rapid screening, the detection workload is heavy and other problems. In addition, chromatography also has problems such as expensive instruments, easy contamination of chromatographic columns, high analysis cost and unsuitability for on-site detection with large fluidity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Xinfumite Cream

[0037] The Xinfufenling of the present embodiment is a cream, and the specification is 15g.

[0038] In this embodiment, whether metronidazole is contained in the Xinfufenling is rapidly detected, and the specific steps are as follows:

[0039] (1) Take 0.5g of Xinfumianling cream and add it into a 10ml disposable syringe, draw 2ml of 3% sulfuric acid solution with the syringe, replace the needle with a 0.45μm organic phase filter, shake vigorously to mix;

[0040] (2) Take 4ml of petroleum ether II and add it to the above-mentioned mixed liquid, shake vigorously to extract, and let stand to separate layers;

[0041] (3) Take the lower layer of acid solution as the test solution and put it in a test tube, add 30 mg of sodium hydroxide solid reagent, and the solution will immediately appear purple, then it can be judged that the present embodiment Xinfufengling contains metronidazole.

Embodiment 2

[0042] Example 2 Yusen Acne Fear Detoxification Acne Essence

[0043] The Yusen Acne and Acne Detoxification Essence of this embodiment is a water preparation with a specification of 30ml.

[0044] In this example, a rapid detection of whether metronidazole is contained in the Yusen Acne Fear Detoxification and Acne Removing Essence is carried out, and the specific steps are as follows:

[0045] (1) Take 3ml of Yusen acne anti-acne detox essence and add it into a 10ml disposable syringe, draw 2ml of 3% sulfuric acid solution with the syringe, replace the needle with a 0.45μm organic phase filter, shake vigorously to mix;

[0046] (2) Take the above-mentioned mixed liquid as the test solution, put it in a test tube, add 30 mg of sodium hydroxide solid reagent, and the solution will immediately appear purple, then it can be judged that the Yusen Acne Fear Detoxification and Acne Removing Essence in this embodiment contains Metronidazole.

Embodiment 3

[0047] Example 3 Gynecological Qianjin Suppository

[0048] The gynecological Qianjin suppository of this embodiment is a disinfectant, with a specification of 6 pieces / box.

[0049] In this embodiment, whether metronidazole is contained in the gynecological Qianjin suppository is rapidly detected, and the specific steps are as follows:

[0050] (1) Take 1 gynecological Qianjin suppository in a test tube, add 3ml of 3% sulfuric acid solution, heat in a water bath until the suppository dissolves, draw the solution into a 10ml disposable syringe, replace the needle with a 0.45μm organic phase filter, shake vigorously to mix uniform;

[0051] (2) Add petroleum ether II 4ml into the above-mentioned mixed liquid, shake vigorously for extraction, and let stand to separate layers;

[0052] (3) Take the lower layer of acid solution as the test solution and put it in a test tube, add 30 mg of sodium hydroxide solid reagent, and the solution will immediately appear purple, indicating ...

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Abstract

The invention discloses a rapid detection method and a detection reagent kit for metronidazole and application thereof in detecting the adding of the metronidazole in cosmetics and disinfectants. The detection method comprises the following steps: firstly, performing acid dissolution and enrichment on a sample to be tested, and then further purifying and separating the sample by organic solvent; and finally developing color development by alkali, and judging whether the sample contains the metronidazole according to the change of the color. The detection method can rapidly detect that whether the metronidazole is added in the cosmetics and the disinfectants; and the detection method has simple and convenient operation, does not need large-scale instruments, and has low detection cost. The method adopts two-step purification of acid and the organic solvent, so that the metronidazole is concentrated and enriched to ensure the correction rate of alkali color development. An alkali color development reaction of the invention adopts sodium hydroxide solid, utilizes the heat released by dissolving sodium hydroxide to reach the temperature needed by the color development reaction, saves water bath equipment, and is more favorable for the fluidity detection of basic level field.

Description

technical field [0001] The invention relates to a detection method of metronidazole, in particular to a rapid detection method of metronidazole, a detection kit and an application in detecting the addition of metronidazole in cosmetics and disinfectants. Background technique [0002] Metronidazole (English name is Metronidazole, MNZ), trade names are metronidazole, metronidazole, metronidazole, metronidazole. The chemical name of metronidazole is 2-methyl-5-nitroimidazole-1-ethanol, and the molecular formula is C 6 h 9 N 3 o 3 . It is a kind of nitroimidazole drug, its nitro group is reduced to amino group in anaerobic environment to show broad-spectrum anti-anaerobic bacteria and antiprotozoal effect. It is mainly used clinically to prevent and treat infections caused by anaerobic bacteria, such as respiratory tract, digestion Tract, abdominal cavity and pelvic pus infection, skin soft tissue, bone and bone joint infection, endocarditis, sepsis and meningitis caused by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78C07D233/94
Inventor 李杨杰蒋忠军
Owner 广东省药品检验所
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