Method for sterilizing air bacterial filter for fermentation tank and fermentation tank provided with air bacterial filter

A sterilization method and fermentation tank technology, applied in the direction of sterilization method, bioreactor/fermentation tank combination, specific-purpose bioreactor/fermentation tank, etc., can solve complex, miscellaneous bacteria pollution, and impure fermentation products and other problems, to achieve the effect of simple structure, easy operation, and simple sterilization operation

Inactive Publication Date: 2009-09-30
王相刚
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermenters that are commonly used today are without exception equipped with an air filter outside the fermenter, which is connected to the fermenter through pipes and valves. In order to ensure complete sterilization, many measures have been taken, but complicated pipe connections, repeated switching valves Complicated operation, if there is a problem in one link, the sterilization will not be complete, the air entering the fermentation tank will be polluted with bacteria, and the fermentation product will be impure
Such fermented products will seriously endanger human health when used in pharmaceuticals and food, but when used for fungal cultivation, they will not grow fruit bodies when used as strains. Therefore, such fermented products cannot be used and scrapped, resulting in a great waste of manpower and material resources. waste

Method used

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  • Method for sterilizing air bacterial filter for fermentation tank and fermentation tank provided with air bacterial filter
  • Method for sterilizing air bacterial filter for fermentation tank and fermentation tank provided with air bacterial filter

Examples

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Embodiment 1

[0015] Embodiment 1: the sterilizing method of air filter for black fungus liquid fermentation

[0016] Use a fermenter with an air filter installed above the fermentation liquid, fill the tank with black fungus liquid fermentation culture liquid, close the valve, and sterilize the fermenter, maintain it at 123°C for 45 minutes, and complete the sterilization of the air filter at the same time . Cool immediately, and when the temperature drops to 25°C, inoculate black fungus strains, open the valve to ventilate, and start fermentation; ferment for 72 hours, and when the fermentation is over, close the valve.

Embodiment 2

[0017] Embodiment 2 see figure 1 . The fermenter of the present invention comprises a fermenter 1, an air filter 3, a pipeline 4, and a valve 5. The fermenter is equipped with an air filter, and the air filter is installed in the cavity 7 above the fermented liquid 2, and the pipeline Vertical to the tank wall of the fermenter, one end of the pipeline communicates with the valve outside the fermenter, and the other end communicates with the air filter of the fermenter. The air hole 6 at the upper end of the air bacteria filter is connected to the required device according to the different bacteria or the needs of the application.

Embodiment 3

[0018] Embodiment 3: see figure 2 , the fermentor described in embodiment 2, its air bacteria filter is installed in the interlayer 8 outside the fermenter, and described pipeline is perpendicular to the outer wall of the interlayer, and the air hole 6 of the air bacteria filter communicates with the cavity in the fermenter.

[0019] The air bacteria filter used in the embodiment of the present invention is a commercially available air bacteria filter.

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Abstract

The invention relates to a method for sterilizing an air bacterial filter for fermentation and a fermentation tank provided with the air bacterial filter. The method for sterilizing the air bacterial filter for the fermentation comprises that: the air bacterial filter is arranged in a space above the fermentation liquid in a fermentation tank or in an interlayer of the fermentation tank or in a connected hopper of a fermentation tank body, an air inlet valve outside the tank body is connected to the air bacterial filter through a pipe in the tank, and the air bacterial filter is sterilized when the fermentation tank is sterilized. The fermentation tank of the invention comprises the fermentation tank (1), the air bacterial filter (3), the pipe (4) and the valve (5), and is characterized in that the fermentation tank is provided with at least one air bacterial filter, the air bacterial filter is arranged in a cavity (7) above the fermentation liquid (2), the pipe is vertical to the tank wall of the fermentation tank, one end of the pipe is communicated with the valve outside the fermentation tank, and the other end of the pipe is communicated with the air bacterial filter of the fermentation tank. The method has the advantage of simple operation, reduces link, and thoroughly ensures the sterilization.

Description

technical field [0001] The present invention relates to a method for sterilizing an air bacteria filter for a fermentation tank and a fermentation tank equipped with an air bacteria filter Background technique [0002] Liquid fermentation technology is widely used in food, pharmaceutical, fungal cultivation and other fields. One of the keys of this technology is the fermentation device, and a good fermentation device must not only ensure the realization of the fermentation process, but also ensure that the fermentation is carried out under sterile conditions. In order to ensure a sterile environment for fermentation, the fermenter, air filter device, and liquid delivery device should be sterilized before fermentation. The fermenters that are commonly used today are without exception equipped with an air filter outside the fermenter, which is connected to the fermenter through pipes and valves. In order to ensure complete sterilization, many measures have been taken, but com...

Claims

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Application Information

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IPC IPC(8): A61L2/04C12M1/12C12M1/08A01G1/04
CPCC12M37/02
Inventor 王相刚李玉陈善殊缪元霞王鑫王斌王臻陈重王相明王庆文薛红凡刘显义张腾霄
Owner 王相刚
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