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Method for detecting astragalus glucoside content in Chinese angelica tonifying blood oral liquid

A technology for the determination of astragaloside IV, which is applied in the field of determination of active ingredients in traditional Chinese medicine preparations, and can solve problems such as complicated separation and purification, asymmetric peak shapes, and poor reproducibility of experimental results.

Active Publication Date: 2009-10-07
郑州市协和制药厂
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AI Technical Summary

Problems solved by technology

[0006] The original quality standard of Angelica Buxue Oral Liquid adopts thin-layer chromatography scanning method. During the experiment, it was found that the thin-layer chromatography scanning method was greatly disturbed by the external environment, the reproducibility of the experimental results was poor, and the separation and purification were complicated.
Since the astragaloside IV in Danggui Buxue Oral Liquid already exists in the liquid sample, its determination is different from that of the crude drug and its preparation, and the conventional method is directly changed to the high performance liquid phase-evaporative light scattering detection method, and the sample is extracted by n-butanol- Ammonia test solution was washed and injected. The results showed that there were many impurity peaks in the chromatogram, the peak shape was asymmetrical, and the reproducibility of the separation was poor.

Method used

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  • Method for detecting astragalus glucoside content in Chinese angelica tonifying blood oral liquid
  • Method for detecting astragalus glucoside content in Chinese angelica tonifying blood oral liquid
  • Method for detecting astragalus glucoside content in Chinese angelica tonifying blood oral liquid

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preparation example Construction

[0046] (2) Preparation of reference substance solution: Weigh an appropriate amount of astragaloside IV reference substance, add methanol to prepare a solution containing 0.4 mg of astragaloside IV in every 1 ml of solution;

[0047] (3) Preparation of the test solution: measure 10 ml of the test product, put it in a separatory funnel, add water-saturated n-butanol to extract 4 times, shake 200 to 250 times each time, use 20 ml of n-butanol each time, combine Four times of n-butanol extract; wash the above obtained n-butanol extract twice with ammonia test solution, take 20ml of ammonia test solution each time, and discard the ammonia solution after the first ammonia test solution is completely separated. ; When washing the ammonia test solution for the second time, it needs to stand still for 2 to 4 hours, and then discard the ammonia solution; take the obtained n-butanol extract, evaporate to dryness, add methanol to dissolve the residue and transfer it to a 5ml measuring bot...

example 1

[0050] Example 1. Get 6 parts of Angelica Buxue Oral Liquid samples, each part of 10ml, each precision add 2ml of Astragaloside IV reference solution (concentration is 0.413mg / ml), prepare by the method determined above, press the selected chromatographic conditions , because the temperature is a range value, 35°C is selected as the temperature of the drift tube this time, each 10ul sample is taken for measurement, and the recovery rate is calculated according to the result. The results are shown in the table below.

[0051] Sample recovery test results

[0052]

example 2

[0053] Example 2. Get 6 parts of Angelica Buxue Oral Liquid samples, each part of 10ml, each precision add 5ml of astragaloside reference substance solution (concentration is 0.413mg / ml), prepare by the method determined above, press the selected chromatographic conditions , because the temperature is a range value, 45°C is selected as the temperature of the drift tube this time, and 10ul samples are taken for each measurement, and the recovery rate is calculated according to the results. The results are shown in the table below.

[0054] Sample recovery test results

[0055]

[0056] The measurement results from the above two examples show that by adding different control amounts of astragaloside IV, good recovery rates have been obtained, indicating that the determined content determination method can accurately determine the content of Danggui Buxue Oral Liquid. Moreover, through further investigation of the determined drift tube temperature within the range of 35°C-45°...

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Abstract

The present invention discloses a method for detecting astragalus glucoside content in Chinese angelica tonifying blood oral liquid. The method uses butyl alcohol for extracting ammine test solution abstersion, uses liquid phantom high-efficient liquid phantom-evaporation light scattering detecting method for detecting content, and confirms some important parameters in detecting. A detecting result proves that a chromatogram obtained by the method has smooth and steady baseline, good peak shape for reaching baseline separation. Sensitivity, stabilizing ability and reproducibility also can conform to requirement for detecting content, and has simple operation. The method is an efficient analytical method for detecting astragalus glucoside content in Chinese angelica tonifying blood oral liquid.

Description

technical field [0001] The invention relates to a method for determining active ingredients in traditional Chinese medicine preparations, in particular to a method for determining astragaloside IV in Angelica Buxue Oral Liquid. Background technique [0002] Danggui Buxue Oral Liquid is a prescription preparation produced by Zhengzhou Xiehe Pharmaceutical Factory. It is the first four new drugs for therapeutic Qi and blood in China. It is approved by the National Committee for the Protection of Traditional Chinese Medicine as a national protected product of traditional Chinese medicine and is exclusively produced nationwide. The oral liquid invigorates qi and nourishes blood, and is suitable for deficiency of both qi and blood. It is used for anemia caused by malnutrition, weak spleen and stomach, and anemia caused by gynecological diseases; it also has the effect of beauty and beauty. Since the Angelica Buxue Oral Liquid is a liquid preparation, the drug is absorbed quickly...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/74
Inventor 李建中
Owner 郑州市协和制药厂