Non-syphilitic leptospira experimental level reaction device and use method thereof

A technology of Treponema pallidum and a reaction device is applied in the field of designing medical serology experimental devices, and can solve the problems of large error in reaction time control, trouble for individuals and society, waste of public health resources and the like

Inactive Publication Date: 2009-10-14
上海市皮肤病性病医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently used instruments mainly have the following defects: ① shaking and jitter during rotation, ② large error in speed frequency accuracy, ③ large error in response time control, ④ lack of humanized display and error correction functions, ⑤ lack of measures to control biological pollution and quality
Just like manual shaking, it is the direct cause of test errors. The test results of samples from the same patient in different laboratories are not comparable, which not only wastes public health resources, but also brings troubles to individuals and society.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0008] Non-Treponema pallidum experimental level reaction device and its use method: According to the principle of VDRL qualitative experiment, the preset conditions of the reaction device are 180rmp and 4 minutes. Take 50ul of serum and place it in the ring of the test card, and evenly coat the entire circle; gently shake the reagent antigen suspension, accurately add 1 drop of antigen (about 18ul) to each specimen, open the upper cover of the device, and react Put the card on the horizontal rotating disk, put down the cover, press the "Start" button to start timing, when the reaction is close to the end, there will be audio and light flashing prompts, and the digital display time, when the experiment is over, the alarm will continue until the "Stop" button is pressed. Immediately observe the results under a microscope and record. If there is a positive result in the qualitative test, take 50ul of normal saline serum and put it in several card rings, take 50ul of serum and mi...

Embodiment approach 2

[0010] Non-Treponema pallidum experimental horizontal reaction device and its use method: According to the principle of RPR / TRUST qualitative experiment, the preset conditions of the reaction device are 100rmp and 8 minutes. Take 50ul of serum and place it in the ring of the test card, and evenly coat the entire circle; gently shake the reagent antigen suspension, accurately add 1 drop of antigen (about 18ul) to each specimen, open the upper cover of the device, and react Put the card on the horizontal rotating disk, put down the cover, press the "Start" button and start timing. When the reaction is close to the end, there will be audio and light flashing prompts, and the number will display the time. When the experiment is over, the alarm will continue until the "Stop" button is pressed. The results were immediately observed visually and recorded. If there is a positive result visible to the naked eye during the qualitative experiment, take 50ul of normal saline serum and put...

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PUM

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Abstract

The invention designs a medical serology experiment device and a use method thereof, in particular a non-syphilitic leptospira experimental level reaction device and a use method thereof. Aiming at overcoming the defects existing in the prior art and method, the invention provides a non-syphilitic leptospira experimental level reaction device with standardized operation and a use method thereof. The non-syphilitic leptospira experimental level reaction device is characterized by comprising an electro-motor, a mechanical transmission box, an eccentric rotation axis connection fulcrum, a rotatable horizontal plate, an electronic control part, a key-press and an acousto-optic digital display panel and particularly characterized by comprising a transparent or semi-transparent outer cover which prevents dust, pollution and dryness, a detachable horizontal rotating plate, a mechanical transmission part box body, an electronic control assembly, related technical requirements and cautions for sound, lighting and digital display, and the combination of routinization and simplification preset memory. When in use, the non-syphilitic leptospira experimental level reaction device can be reasonably assembled and operated according to the requirement of experiment purpose.

Description

technical field [0001] The invention designs a medical serological experiment device and a use method, and specifically designs a non-treponemal test horizontal reaction device and a use method. Background technique [0002] Serological tests are the main means of diagnosing syphilis. Non-Treponema pallidum experiments represented by Venereological Research Experimental Research Test (VDRL), Rapid Plasma Reaction Test (RPR) or Toluidine Red Unheated Serum Test (TRUST), the principle of the experiment is easy to understand, and the condition of VDRL experiment is 180rmp±≤5rmp The reaction time is 4 minutes ± ≤ 3 seconds, and the conditions for RPR and TRUST experiments are 100rmp ± ≤ 5rmp for 8 minutes ± ≤ 5 seconds, which is a commonly used test item in medical institutions. The nontreponemal test is mainly used for the screening of syphilis and the judgment of curative effect [Guidelines for Laboratory Diagnosis of Sexually Transmitted Diseases 2007.7-8.], the correctness ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/571G01N35/02
Inventor 顾伟鸣
Owner 上海市皮肤病性病医院
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