Propagation method for keeping cabbage RGMS male sterile line

A male sterile line and cabbage technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as incomplete hybridization rate of breeding plants, achieve overcoming the cumbersomeness of normal breeding, improve breeding effects, and maintain constant Breeding effect

Inactive Publication Date: 2009-10-28
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method not only effectively solves the large amount of manpower and financial resources invested in the removal of fertile plants during large-scale seed production, but also reduces the cost of seed production; at the same time, it eliminates the problem of incomplete removal of fertile plants or untimely removal of the first flowering stage to reduce the hybridization rate. problem, improved hybrid seed quality

Method used

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  • Propagation method for keeping cabbage RGMS male sterile line
  • Propagation method for keeping cabbage RGMS male sterile line
  • Propagation method for keeping cabbage RGMS male sterile line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] In this example, adult plants (large plants) of the male sterile dual-purpose line of cabbage were selected, and the cauline leaves of the male sterile plants were selected according to the indicator traits of stamen degeneration of flowering flowers, that is, the new first-generation cabbage hybrid combination "Xia Kang The female parent of 69”—the adult plant (large plant) of the nuclear male sterile RGMS male sterile line RGMS05-632M5168 was used as the material.

[0030] 1) Cultivation of adult (large) bolting plants of the RGMS male sterile line

[0031] The seeds harvested from the sterile plants of the RGMS05-632M5168 line were sown in the open field in early July to raise seedlings. When the seedlings had 6-7 true leaves, they were planted in the field for normal cultivation and management. Before the winter freeze (early and mid-November), dig up the roots of the RGMS05-632M5168 series with tight balls, and plant them in the prepared cellar in advance. In winte...

Embodiment 2

[0044] In this example, the cauline leaves of the semi-adult sterile plants of the male sterile line are selected, that is, the female parent-nuclear male sterile RGMS male sterile line RGMS05-632M5168 of the new cabbage hybrid combination "Yuexiawang" The stem leaves of the semi-adult bolting plants are used as materials.

[0045] 1) Cultivation of RGMS male sterile line semi-adult bolting plants

[0046] Seeds harvested from the sterile plants of the RGMS05-632M5168 line were sown in the open field in mid-August, and when the seedlings had 6 to 7 true leaves, they were planted in the field in mid-to-late September for normal cultivation and management; The plants of the Baoqiu RGMS05-632M5168 line are cultivated to keep warm, irrigated in winter, and the plants survive the winter in the open field. After the temperature rises in the spring of the following year, weeding in the field, building insect-proof gauze sheds on the plants, topdressing and watering to promote boltin...

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Abstract

The invention discloses a propagation method for keeping a cabbage RGMS male sterile line, which comprises the following steps: selecting a scape leaf blade of the cabbage male sterile line, inoculating the scape leaf blade on an adventitious bud differentiation induction culture medium for cultivation, and when adventitious buds grow to a height of between 2.0 and 4.0 centimeters, transplanting the adventitious buds to an adventitious root differentiation induction culture medium for cultivation to obtain a complete regrowth seedling; transplanting the regrowth seedling to cultivating soil for cultivation; when the seedling grows to have 6 to 8 true leaves, hardening off the seedling adaptively for 3 to 5 days and then planting the seedling permanently in a field (an open ground), wherein the survival rate of the seedling is over 95 percent. By adopting the method, the breeding effect of the cabbage can be improved, and the normal breeding of the male sterility of the cabbage is ensured and enlarged. The method overcomes the complexity of the normal breeding of the male sterility of the cabbage, not only keeps the sterile line but also greatly improves the propagation coefficient, and provides guarantees for mass seed production.

Description

technical field [0001] The invention belongs to the technical field of utilization of plant heterosis, and relates to a method for maintaining and propagating male sterile lines in cabbage recessive genetic male sterility (RGMS) by cultivating stem leaf tissue of biennial plants . Background technique [0002] The discovery and utilization of male sterile lines of vegetable crops is of great significance in the utilization of heterosis, thus expanding the application range of heterosis in crops and achieving a 100% hybridization rate of hybrids, which is beneficial to improving seed quality and crop production, and obtaining more Great economic benefits played an important role. Cabbage is one of the important vegetable crops. It is widely planted all over the country. It has high nutritional value, multi-stubble cultivation, and annual supply. All cultivated varieties in production use first-generation hybrid seeds. There are two ways to obtain the first-generation hybrid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张恩慧程永安许忠民杨安平马青山王小艳许念芳张高翔刘辉
Owner NORTHWEST A & F UNIV
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