Abamectin batch fermentation optimizing process

Abstract

The present invention provides an abamectin batch fermentation optimizing process, which is improved on the basis of the process for producing the abamectin by the streptomyces avermitilis batch fermentation, wherein, the carbon source proportion in the fermentation solution is 8-11 % during fermentation initial stages, when the fermentation is performed for 20-30 hours, the mixed solution containing glucose and starch is added, so that the carbon source proportion in the fermentation solution keeps 8-11 %; after fermenting 50-60 hours, the adding of the mixed solution containing glucose and starch is stopped, the sterile water is added, the added volume of the sterile water is 2-10 % of the total volume of the added primary fermentation solution; after fermenting 175-185 hours, 15%-20% fermentation solution is added, the residual fermentation solution is added with nutrient solution, the carbon source proportion in the fermentation solution keeps 1-4 %, the fermentation period is completed. The abamectin batch fermentation optimizing process provided by the invention improves the utilization ratios of the fermentation process, the fermentation equipment and the raw materials mainly by the optimization of the material compensating process.

Description

technical field [0001] The invention relates to a batch fermentation process of abamectin. Background technique [0002] Abamectin is a low-toxicity, high-efficiency, high-selection, green, environmental-friendly, bio-sourced insecticide, which completely solves the problems of high toxicity, resistance and environmental impact of traditional pesticides. As abamectin, one of the most effective insecticides in the world, the key to improving its production level lies in the optimization of abamectin fermentation process. At present, the fermentation process of abamectin mostly adopts the batch fermentation method, and the actinomycete called Streptomyces avermanii is used as the production strain, and it is cultivated under aerobic conditions. The culture medium in the cultivation adopts corn starch as the main Carbon source, combined with bean cake powder, peanut powder and yeast extract as nitrogen source, and adding a small amount of inorganic salts for fermentation, the ...

AI Technical Summary

Problems solved by technology

With the doubling of the medium concentration, although the yield has also increased, it is followed by incomplete utilization of the medium, a decrease in the fermentation yield per unit of dry matter, a decrease in the utilization rate of fermentation equipment, and an increase in the COD concentration of fermentation sewage. A series of problems such as increased difficulty

[0004] At present, although there are researches on abamectin fermentation feeding technology, in the middle and later stages of abamectin fermentation, the production is increased by feeding or supplementing glucose solution, but the effect is not obvious, and only part of the tank volume can be increased, while Problems such as incomplete utilization of medium, decreased fermentation yield per unit dry matter, and decreased utilization of fermentation equipment have not been completely resolved

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