A kind of high-density fermentation method of recombinant human interferon alpha 2b

Abstract

The invention provides a high-density fermentation method of recombinant human interferon α2b, which controls the feed feeding of nutrients through online detection of glucose concentration, maintains the respiratory quotient between 0.9 and 1, and controls relatively constant dissolved oxygen, so as to achieve The purpose of maintaining the low level of organic acid is to realize the high-density fermentation of recombinant human interferon α2b.

Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to a high-density fermentation method of recombinant human interferon α2b. Background technique

[0002] The current level of high-density fermentation in my country is far behind that of foreign countries. The concentration of bacteria after fermentation and the output of products are very different from the international level. For example, the fermentation of recombinant E. coli producing recombinant human growth hormone in China can reach The OD600 of the liquid is 50-60, while abroad can reach the level of 120. The output of the target product is only 0.4g / L in China, while the foreign country can reach the level of 1.1g / L. The high-density fermentation of Pichia pastoris has more advantages. It can grow to an OD value above 500 in a synthetic medium, and the volume of the bacteria can account for more than half of the volume of the fermentation broth.

[0003] In terms of high-densi...