Device for collecting root exudate in situ

Abstract

The invention discloses a device for collecting root exudates in situ, comprising an incubator, a bracket, an illumination cultivation light, a straight glass tube, a peristaltic pump, a nutrients bottle, a resin column, a surge flask and a collection bottle, wherein, cultivation medium is arranged in the incubator, the outer surface of the incubator is covered with a plastic thin film, the upper end of the incubator is provided with a sealing board, and the side of the incubator is inserted with a thermometer; the lower end of the incubator is provided with a through hole which is sealed by a rubber plug, and glass wool is arranged on the rubber plug; the incubator is fixed by the bracket arranged on a base, an adjustable bracket is arranged on the bracket, and the upper end of the adjustable bracket is provided with the illumination cultivation light; a porous circular glass tube is arranged in the medium in the incubator, and the bottom of the porous circular glass tube is connected with the straight glass tube; the straight glass tube extends out of the bottom rubber plug and is inserted into the nutrients bottle via the peristaltic pump; one resin column is used for collecting the glass tube, one end of the glass tube passes through the rubber plug to extend into the glass wool in the incubator, and the other end is connected with the resin column; the lower end of the resin column is connected with the glass tube, and the other end of the glass tube is inserted into the surge flask; and the collection bottle is connected with the surge flask via the collecting tube. The invention can truly reflect the characteristics of root exudates when plants grow in soil environment in laboratories.

Description

Technical field: [0001] The invention relates to a research device for phytoremediation of heavy metal polluted soil, in particular to a device for continuously collecting plant root exudates under heavy metal stress in situ under laboratory conditions. Background technique: [0002] At present, with the development of phytoremediation technology for heavy metal-contaminated soil, the research on plant root exudates has attracted much attention. The characteristics and changes of its components directly affect the growth of plants and the bioavailability of heavy metals in soil. [0003] It is not feasible to collect specific plant root exudates under heavy metal pollution conditions in field trials. Moisture, temperature, nutrients, oxygen content, pH, etc. in the soil change irregularly, and the growth of plants varies greatly from time to time and from place to place. In addition, the existence of a large number of microorganisms in the soil will inevitably affect the gr...

AI Technical Summary

Problems solved by technology

However, liquid culture is different from the soil environment. There is no extrusion problem in the soil environment for plant roots in the liquid. At the same time, the oxygen content in the soil also greatly affects the exudation process of plant roots. Therefore, liquid culture can not collect root exudates. Truly reflect the characteristics of root exudates when plants grow in soil environment

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