Method for preparing composition rich in alpha 1-antitrypsin from supernatant of a composition A+I by improved K-N method

Abstract

The invention belongs to the field of plasma protein preparation, and in particular relates to a method for preparing a composition rich in alpha 1-antitrypsin from supernatant of a composition A+I by an improved K-N method. The invention adopts the technical scheme that: the composition rich in the alpha 1-antitrypsin is prepared by adjusting five parameters, namely the protein concentration, pH, ethanol concentration, conductivity and temperature of the supernatant of the composition A+I by the improved K-N method. The method has simple operation, can greatly reduce investment on equipment for preparing the alpha 1-antitrypsin, greatly reduces operating volume, overcomes the defect that the prior art has high requirement on the equipment, needs a large-scale chromatographic system and supporting facilities, has long operating time and the like, improves the purification efficiency, and has good application prospect.

Description

technical field [0001] The invention belongs to the field of plasma protein preparation, and in particular relates to the preparation of α-rich 1 - A method for the antitrypsin (AAT) component. Background technique [0002] alpha 1 - Antitrypsin (Alpha-1 antitrypsin AAT). The molecule is composed of 394 amino acids, glycoprotein with a molecular weight of 52,000. It exists in two forms: natural active and denatured inactive. It is produced by the liver. Its main function is to inhibit the activity of trypsin and elastase of neutrophils. It can also inhibit Chymotrypsin, plasmin, etc. It is one of the most powerful enzyme inhibitors in the human body. Congenital deficiency of AAT such as gene mutation or acquired factors such as long-term smoking or bacterial infection will cause the inactivation of AAT active center, which will lead to functional deficiency of AAT and then cause the imbalance of protease and anti-protease system in the body, and the ability of lung tissu...

AI Technical Summary

Problems solved by technology

But it also caused some problems. Some important plasma protein products such as α 1 - Difficulty in preparing antitrypsin, etc.

There are some manufacturers and researchers in the world, such as Burnouf et al "Biochemical and Biological Properties of an α 1 -antitrypsin Concentrate" Vox Sang52: 291-297 (1987) Component A or component A+I supernatant of Kistler and Nitschmann method is used for separation and purification through chromatographic column α 1 -Antitrypsin, this method has defects such as high requirements for equipment, large-scale chromatography system and supporting facilities, and long operation time. This has been a problem that has plagued those skilled in the art for more than 20 years.

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