Prostatic cancer related gene and application thereof

A prostate cancer and gene technology, applied in the biological field, can solve problems such as normal cell damage and cancer cell toxicity

Inactive Publication Date: 2010-01-27
SHANGHAI INST OF PLANNED PARENTHOOD RES +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It is known that a variety of molecules are overexpressed in prostate cancer tissues, and thus identified as prostate cancer markers or therapeutic targets. However, most of these molecules are also overexpressed in other major organs. Drugs targeting these molecules may be effective on cancer cells. Is toxic but can also cause damage to normal cells in other organs

Method used

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  • Prostatic cancer related gene and application thereof
  • Prostatic cancer related gene and application thereof
  • Prostatic cancer related gene and application thereof

Examples

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preparation example Construction

[0079] The preparation method of the small interfering RNA is not particularly limited in the present invention, including but not limited to: chemical synthesis method, in vitro transcription method and the like. It should be understood that those skilled in the art can conveniently prepare or express the small interfering RNA in various ways after knowing the sequence of the siRNA provided by the present invention. For example, in a preferred embodiment of the present invention, the small interfering RNA is chemically synthesized.

[0080] Small interfering RNA can be prepared in the form of double-stranded nucleic acid, which contains a sense strand and an antisense strand, and these two strands form a double strand only under hybridization conditions. A double-stranded RNA complex can be prepared from separate sense and antisense strands. Thus, for example, complementary sense and antisense strands are chemically synthesized and subsequently hybridized by annealing to pro...

Embodiment 1

[0109] Example 1. Cell culture

[0110] Prostate cancer cell lines LNCaP cells, PC-3, DU145, CL-1 and immortalized prostate stromal cells WPMY-1 were purchased from ATCC. LNCaP cells were cultured in DMEM medium containing 5% FBS and 100 U / ml penicillin and streptomycin. Other cell lines were cultured in RPMI 1640 medium containing 10% FBS and 100U / ml penicillin and streptomycin. All cells were kept at 37°C with 5% CO 2 cultured in an incubator.

Embodiment 2

[0111] Example 2.MPSS detects the expression of NDRG3 in human tissue

[0112] Extract various human tissues (see figure 1 ) total RNA, mRNA was synthesized into cDNA double strands with biotin-labeled oligonucleotide primer (oligo-dT); Digested cDNA fragments were purified; synthesis contained 1.67 × 10 7 A tag vector for a 32bp oligonucleotide fragment; clone the purified cDNA fragment into the tag vector, and amplify the insert by PCR primers on the tag vector. Enzyme digestion and digestion of the PCR product to generate a product containing a cDNA fragment connected to a 32bp tag; the cDNA fragment is connected to the microsphere through tag and anti-tag hybrid connection, and each microsphere can also carry 10 4 -10 5 identical cDNA copies.

[0113] Further digest the cDNA template bound on the microsphere, and use different oligonucleotide adapters to connect with the cDNA template attached to the microsphere. In addition, the 3' end of each adapter also has a fluor...

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Abstract

The invention discloses a prostatic cancer related gene and application thereof. The gene is an N-myc downstream regulation type III gene (NDRG3). The NDRG3 has high expression in prostatic cancer tissues, and the over-expression of the gene can promote the proliferation of prostatic cancer cells and reduce the biological effect on the prostatic cancer cells. The reduction of the expression of the protein of the NDRG3 can obviously inhibit the growth of the prostatic cancer cells. Thus, the NDRG3 can be used as a novel target molecule for diagnosing and treating the prostatic cancer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular, the invention relates to prostate cancer-related N-myc downstream regulation type 3 gene (NDRG3) and application thereof; an antagonist for NDRG3 gene or protein and application thereof. Background technique [0002] Prostate cancer is a common disease among men in European and American countries. In the past 20 years, the increase in the incidence of prostate cancer in China has also ranked first among all kinds of tumors. When prostate cancer patients are diagnosed, about half of the cases are in the advanced stage, and most of the cases have cancer cell metastasis, which not only loses the chance of early radical cure, but also the prognosis of treatment at this stage is very unsatisfactory. Therefore, prostate cancer has become a major disease that endangers the health of elderly men. The molecular pathological mechanism of prostate cancer is intricate. Not only polygenes are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61K31/7088G01N33/53A61P13/08A61P35/00
Inventor 李润生刘新垣林标扬顾锦法王伟群李玉华范钧锴李扬
Owner SHANGHAI INST OF PLANNED PARENTHOOD RES
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