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Novel microorganism

A technology of microorganisms and deposit numbers, applied in the field of new microorganisms capable of producing hydrogen, can solve problems such as low hydrogen production rate

Inactive Publication Date: 2011-11-23
SAPPORO BREWERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the hydrogen production rate was low due to problems such as the mixing of bacteria in the past hydrogen fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 In Example 1, hydrogen fermentation was carried out at various pHs. For this microorganism, PEH9 strain was used. In addition, as a control, the Thermoanaerobacterium pyrolyticum saccharolyticum PEH8 strain and the ATCC7956 strain different from the PEH9 strain were used. The medium used contains 1.0% (w / v) xylose, 0.4% (w / v) yeast extract (Jidong Pharmaceutical), 0.25% (w / v) MOPS, 0.091% (w / v) K 2 HPO 4 , 0.03% (w / v) NaH 2 PO 4 , 0.02% (w / v) MgCl 2 , 0.01% (w / v) CaCl 2 , 0.03% (w / v) (NH 4 ) 2 SO 4 , 0.002% (w / v) FeSO 4 , 0.04% (w / v) L-cysteine, 0.11% (w / v) resazurin in water.

[0032] The process of obtaining PEH9 strain and PEH8 strain is briefly described below. First, the high-temperature anaerobic digestion sludge collected from the sewage treatment facility was domesticated with brewery wastewater for about one month to obtain a hydrogen-fermenting bacteria group that stably produced 50%-60% hydrogen and 40%-50% carbon dioxide. A modified G...

Embodiment 2

[0042] Example 2 In Example 2, the hydrogen production property in the range of pH 5.0-pH 5.2 was confirmed on a 1 L scale. For this microorganism, PEH9 strain was used. The culture medium used 1.0% (w / v) xylose, 2% (w / v) peptone, 0.5% (w / v) yeast extract, 1.0% (w / v) malt extract, 0.3% ( w / v)K 2 HPO 4 , 0.1% (w / v) MgSO 4 , 0.02% (w / v) L-cysteine, 0.2% (w / v) L-malic acid, 0.003% (w / v) oleic acid and 0.1% (w / v) Tween 80 in water. Subsequently, PEH9 strain was inoculated in 500 mL of medium in a 1 L fermenter (BMJ-01, manufactured by ABLE Co., Ltd.), and continuously cultured at a dilution rate of 0.3-0.4 under anaerobic conditions.

[0043] The culture temperature was stabilized at 55°C. The pH of the culture solution was adjusted to the range of pH 5.0-pH 5.2 by automatically adding aqueous sodium hydroxide solution to the culture solution.

[0044] After confirming that the PEH9 strain proliferated stably in the culture medium, the amount of fermented gas produced ever...

Embodiment 3

[0048] Example 3 In Example 3, the hydrogen generating property in the range of pH 5.8-pH 6.0 was confirmed on a 1 L scale. Hydrogen fermentation was carried out under the same conditions as in Example 2 above, except that the pH range was different.

[0049] Figure 5 The results of the hydrogen production performance measurement of the PEH9 strain are shown. Figure 5 , the horizontal axis represents the number of days of culture (day), the left vertical axis represents the amount of fermented gas produced during each culture day (mL / day), and the right vertical axis represents the volume ratio of hydrogen contained in the fermented gas (% ), the white column represents the fermented gas production, and the triangle mark represents the hydrogen volume ratio.

[0050] Such as Figure 5 As shown, the PEH9 strain can stably produce hydrogen-containing fermentation gas for 5 consecutive days. Specifically, the 5-day average fermented gas output is 1676mL / day, the average h...

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Abstract

It is intended to provide a novel microorganism which is suitable for the production of hydrogen. This organism is a strain ofThermoanaerobacterium thermosaccharolyticum under the deposition number FERM BP-10793 or its variant.

Description

technical field [0001] The present invention relates to novel microorganisms, in particular novel microorganisms capable of producing hydrogen. Background technique [0002] Hydrogen has attracted attention as an energy source that can be used in fuel cells and the like. Hydrogen fermentation using hydrogen-producing microorganisms is one of hydrogen-producing methods (for example, Patent Document 1). Patent Document 1: JP-A-4-169178 Contents of the invention [0003] Technical issues to be solved [0004] Microorganisms conventionally used for hydrogen fermentation do not have sufficient hydrogen production ability in the low pH range. In addition, the conventional hydrogen fermentation has a low hydrogen production rate due to problems such as the mixing of bacteria. [0005] In view of this, the inventors obtained new microorganisms suitable for hydrogen production through earnest research, and thus completed the present invention. [0006] Technical solution to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P3/00
CPCC12R1/01C12P3/00C12N1/205C12R2001/01
Inventor 冲泰弘三谷优
Owner SAPPORO BREWERIES
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