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Method for purifying and separating X-fraction polysaccharide from fruit bodies or mycelia of polyporus frondosus

A separation method and mycelium technology are applied in the field of extraction and separation of Grifola frondosa fruiting bodies or mycelium polysaccharide components, which can solve problems such as complicated purification steps, and achieve the effects of high purity and convenient operation.

Active Publication Date: 2010-03-03
ZHEJIANG FANGGE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, those methods described above are not suitable for the preparation of a large amount of medicines and the efficient preparation of health foods from limited resources, because the purification steps of these methods are rather complicated, and the obtained products contain substances that suppress the immunopotentiating activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The extraction of embodiment 1 Grifola frondosa X component (molecular weight is about 28W Dalton)

[0023] Weigh 15Kg of Grifola frondosa fruiting body fine powder, add 500L of water, extract at 100°C for 1h, centrifuge at 8000rpm for 20min, concentrate the supernatant to a density of 1.04, add 3 times the amount of 95% ethanol, stand overnight at 4°C, filter, The obtained precipitate was redissolved by adding 30 times of hot water, centrifuged, and 45% CTAOH was added until no more precipitate was produced, adjusted to PH = 11, kept at 4°C for 48 hours, centrifuged, and the supernatant was discarded to obtain a precipitate; Wash 2 times with 20% acetic acid, 2 times with 90% ethanol, redissolve with 0.5 NaOH solution, centrifuge, deproteinize the supernatant 3 times with sevag method; add ethanol to the supernatant to make the ethanol concentration 65%, Precipitate, redissolve the precipitate with 0.2M NaOH solution, add ethanol to precipitate to make the ethanol conc...

Embodiment 2

[0024] The extraction of embodiment 2 Grifola frondosa X component (molecular weight is about 35W Dalton)

[0025] Weigh 15Kg of Grifola frondosa according to four body fine powder, add 500L of water, extract at 100°C for 1h, centrifuge at 8000rpm for 20min, concentrate the supernatant to a density of 1.04, add 3 times the amount of 95% ethanol, and let stand overnight at 4°C. Filter, add 30 times of hot water to the obtained precipitate to redissolve, centrifuge, add 60% CTAOH until no more precipitate occurs, adjust pH=12, keep at 4°C for 48 hours, centrifuge, discard the supernatant to obtain the precipitate; precipitate with 2 times Wash 2 times with 20% acetic acid by volume, wash 2 times with 90% ethanol, redissolve with 0.5 NaOH solution, centrifuge, and deproteinize the supernatant 3 times by sevag method; add ethanol to the supernatant to make the ethanol concentration 60 %, precipitation, redissolve the precipitation with 0.2M NaOH solution, add ethanol for precipita...

Embodiment 3

[0026] The extraction of embodiment 3 Grifola frondosa X component (molecular weight is about 43W Dalton)

[0027] Weigh 15Kg of Grifola frondosa fruiting body fine powder, add 500L of water, extract at 120°C for 1h, centrifuge at 8000rpm for 20min, concentrate the supernatant to a density of 1.04, add 3 times the amount of 95% ethanol, stand overnight at 4°C, filter, Add 30 times of hot water to the obtained precipitate to redissolve, centrifuge, add 80% CTAOH until no more precipitate occurs, adjust pH=11.5, keep at 4°C for 48 hours, centrifuge, discard supernatant to obtain precipitate; precipitate with 2 times the volume of Wash 2 times with 20% acetic acid, 2 times with 90% ethanol, redissolve with 0.5 NaOH solution, centrifuge, deproteinize the supernatant 3 times with sevag method; add ethanol to the supernatant to make the ethanol concentration 55%, Precipitate, redissolve the precipitate with 0.2M NaOH solution, add ethanol to precipitate to make the ethanol concentra...

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Abstract

The invention relates to a method for purifying and separating X-fraction polysaccharide from the fruit bodies or mycelia of polyporus frondosus, which is characterized in that: crude polysaccharide extracted from the fruit bodies or the mycelia of the polyporus frondosus is subjected to complex reaction and primary purification, and is deproteinized by a sevag method, and then X-fraction pure polysaccharide of the polyporus frondosus is prepared by a graded ethanol precipitation method. The polysaccharide has good effect of reducing blood fat. The crude polysaccharide is obtained by a water extraction and ethanol precipitation method, and is separated and purified according to different natures of a complex compound and the X-fraction polysaccharide to ethanol precipitation concentration.The method has convenient operation, obtains the target product with high purity, and provides a feasible way for producing the X-fraction polysaccharide of the polyporus frondosus on a large scale,and preparing various medicaments and health-care foods.

Description

technical field [0001] The invention relates to a method for extracting and separating effective components of edible fungi, in particular to a method for extracting and separating polysaccharide components of frondosa frondosa fruit body or mycelia. Background technique [0002] Grifola frondosa (Polyporus frondosus) belongs to the phylum Fungi, Basidiomycotina, Phyllomycetes, Polyporaceae, and Polyporaceae. Grifola frondosa is a famous edible and medicinal fungus, which is rich in nutrition and delicious. It is a large fungus that is "edible, tonic, and medicinal, and the whole body is a treasure". Grifola frondosa is flat in nature and sweet in taste, and can be used to treat dysuria, edema, beriberi, liver cirrhosis, ascites, diabetes, high blood pressure, obesity and other diseases. [0003] Known to be extracted from the mycelia or fruiting bodies of Grifola frondosa, consisting of a β-1,6-linked glucose backbone with β-1,3-linked glucose branches or with a β- - Poly...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P3/06A23L1/28A23L1/29A23L1/30A23L1/09A23L29/30A23L31/00A23L33/105
Inventor 徐财泉
Owner ZHEJIANG FANGGE PHARMA
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