Effective portion of conical hydrangea, preparation method thereof, and composition and usage thereof

A technology of effective parts and hydrangea paniculata, applied to medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc.

Active Publication Date: 2010-04-14
北京协和制药二厂有限公司 +1
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pane hydrangea is rich in natural resources, and no toxic reports have been seen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Effective portion of conical hydrangea, preparation method thereof, and composition and usage thereof
  • Effective portion of conical hydrangea, preparation method thereof, and composition and usage thereof
  • Effective portion of conical hydrangea, preparation method thereof, and composition and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1 kg of roots or stems of Hydrangea paniculata were refluxed three times with 95% ethanol for 2 hours each time, concentrated under reduced pressure at 60° C. to obtain extracts. This extract is dissolved in ethanol: tetrahydrofuran: a small amount of water (8:8:1), and the supernatant is mixed with 100-200 mesh 250g silica gel as a sample, dried in a water bath at 60°C, passed through a 100-200 mesh 100g silica gel column, respectively Elute with 3500ml of petroleum ether: acetone (1:1), 2000ml of acetone, and ethanol, collect the acetone eluate, concentrate under reduced pressure at 60°C to obtain each fraction respectively. The acetone fraction (crude fraction) was dissolved with ethanol: tetrahydrofuran: water (7:8:3), passed through an ODS column, and eluted with 10-15% ethanol mobile phase to obtain a hydrangea extract containing more than 50% inosin.

Embodiment 2

[0049] 1 kg of roots or stems of Hydrangea paniculata were refluxed three times with 95% ethanol for 2 hours each time, concentrated under reduced pressure at 60° C. to obtain extracts. This extract is dissolved in ethanol: tetrahydrofuran: a small amount of water (8:8:1), and the supernatant is mixed with 100-200 mesh 250g silica gel as a sample, dried in a water bath at 60°C, passed through a 100-200 mesh 100g silica gel column, respectively Elute with different concentrations of 3500ml petroleum ether: acetone (1:1), 2000ml acetone, ethanol, collect the acetone eluate, concentrate the acetone eluate at 60°C under reduced pressure to obtain the acetone fraction (crude fraction). The acetone part is subjected to macroporous resin column chromatography and eluted with 10-40% ethanol to obtain hydrangea paniculata extract containing more than 50% inosin.

Embodiment 3

[0051] 1 kg of roots or stems of Hydrangea paniculata were refluxed three times with 95% ethanol for 2 hours each time, concentrated under reduced pressure at 60° C. to obtain extracts. This extract ethanol: tetrahydrofuran: a small amount of water (8: 8: 1) dissolves, gets the supernatant and 100-200 order 250g silica gel to mix sample, water bath 60 ℃ of oven dry, carry out silica gel column chromatography (loading amount and silica gel ratio), with CH 3 Cl:MeOH:H 2 O (8:2:0.1) mobile phase was eluted to obtain the hydrangea paniculata extract containing more than 50% inosin.

[0052] Pharmacological experiment

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an effective portion extracted from a conical hydrangea (Hydrangea paniculata sieb), and the preparation method of the effective portion comprises drug composite of the effective portion and the purpose of the effective portion and other drug composite which are used as drugs, and the effective portion is particularly applied in preparing drugs for preventing and / or treating kidney incompetence, high blood pressure and diabetic kidney disease.

Description

[0001] This case is a divisional application, the filing date of the parent case is April 23, 2004, the application number is 200410034068.8, and the invention name is "Effective parts of Hydrangea paniculata, its preparation method, its composition and use". technical field [0002] The present invention relates to the effective part extracted from Hydrangea paniculata, the preparation method of the effective part, the pharmaceutical composition containing the effective part, and the use of the effective part and its pharmaceutical composition as medicine, especially in the preparation of preventive and / or the application of drugs in the treatment of renal insufficiency, hypertension and diabetic nephropathy. Background technique [0003] Chronic kidney disease (including various types of chronic nephritis, diabetic nephropathy and hypertensive renal damage, etc.) is the most common chronic refractory disease. The annual incidence rate of chronic renal failure (CRF) in th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P9/12A61P13/12
Inventor 张东明陈晓光杨敬芝李燕郑旭光
Owner 北京协和制药二厂有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap