Fluorescent nanoparticle and preparation method and application thereof

Abstract

The invention discloses a class of fluorescent nanoparticles and a preparation method and an application thereof. The fluorescent nanoparticle comprises matrix material and fluorescent dye dispersed in the matrix material, wherein the matrix material is the compound composed of hydrophobic polymer main chain of which is hydrocarbon chain, organo-siloxane and organosilicon-polymer, and the fluorescent dye is rare earth complex with photoluminescence performance. The preparation method of the fluorescent nanoparticle comprises the following steps: dissolving rare earth complex, hydrophobic polymer and compound RSi (OR') 3 into organic solvent miscible with water; adding the mixture into aqueous solution containing surfactant; using precipitation and hydrolytic condensation reaction to form nanoparticles. The nanoparticle of the invention has favourable luminous performance and favourable stability, can have a silicon oxide shell and a surface functional group and can be used for bonding biomolecules; biological probes based on the fluorescent nanoparticle have wide application prospects on the aspects of high-sensitivity fluorescence immunoassay, imaging and the like.

Description

technical field [0001] The invention discloses fluorescent nanoparticles and their preparation methods and applications, in particular to a class of fluorescent nanoparticles based on rare earth complex fluorescent dyes and their preparation methods and applications. Background technique [0002] Fluorescent probes based on luminescent rare earth complexes have important application prospects in biosensing, cell and tissue imaging, etc. The main reason is that the sharp line emission of rare earth ions in the visible or near-infrared region and the long lifetime of the luminescent state can reduce the fluorescence interference generated by the biological material itself, which is expected to make the detection with high sensitivity and efficiency, while avoiding the use of radioactive Elements pose a series of problems. [0003] Because the f-f transition of rare earth ions is limited by the electric dipole selection rate, the light absorption ability of rare earth ions is ...

AI Technical Summary

Problems solved by technology

Generally, the dispersion of nanoparticles with hydrophobic polymer as the matrix is ​​not ideal in aqueous solution, and they are prone to agglomeration. However, in many organic solvents, due to the swelling of the polymer, it is easy to cause the leakage of fluorescent molecules in the nanoparticles.

The wettability of the silica matrix and the water-insoluble rare earth complexes is poor. When the silicon oxide formed by the hydrolysis reaction wraps the water-insoluble rare earth complexes to synthesize nanoparticles, it is difficult to form a dense coating layer, which often leads to the formation of fluorescent nanoparticles. low stability

[0007] The inventors of the present invention have used the reported synthesis method of rare earth complex-based fluorescent nanoparticles to wrap the designed and synthesized rare earth complexes with excellent visible light and near-infrared light excitation and luminescence properties to synthesize new rare earth complex-based fluorescent nanoparticles , such as microemulsion polymerization, RSi(OR') 3 Hydrolysis embedding method, etc., but it is difficult to obtain satisfactory results

One of the main reasons is that the ligands of rare earth complexes with excellent visible light and near-infrared excitation and luminescence properties have weak coordination with rare earth metal ions, and the usual complex embedding methods easily lead to ligand exchange reactions and loss of Original excitation and luminescence properties

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