Technique for preparing hermaphrodite scallop interspecific hybrid fingerlings

A technology of scallop and body shape, which is applied in the field of preparation of hermaphroditic scallop interspecific hybrid seedlings, can solve problems such as difficulty in summer and overwintering, inability to breed, and narrow temperature adaptation range of purple scallop, and achieves large heterosis, easy purification, and high fertilization rate high effect

Inactive Publication Date: 2010-05-12
QINGDAO AGRI UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, purple scallops have a narrow temperature adaptation range (8-22°C), and it is difficult to spend summer an

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: After raising the temperature of purple scallops and bay scallops to promote ripening to reach sexual maturity, 18 mature purple scallops and bay scallops were taken respectively, and sperm eggs were obtained after stimulating oviposition and ovulation. The sperm and eggs of two kinds of scallops were cross-fertilized to obtain 4 combined populations of Chunzi (ZZ), Chunhai (HH), Zihai (ZH) and Haizi (HZ), and the fertilization rate of each group was counted. The experimental results showed that the sperm and eggs of purple scallop and bay scallop had higher compatibility, and the fertilization rate of each hybrid group was higher than that of bay scallop, but slightly lower than that of purple scallop selfing group.

Embodiment 2

[0013] Example 2: After obtaining 4 combined populations of pure purple (ZZ), pure sea (HH), purple sea (ZH) and sea purple (HZ) as in Example 1, cultivate them at 22-23 degrees, carefully observe and compare Embryo development in each group. The results showed that the embryonic development of the purple scallop was faster than that of the bay scallop, and the development of the two hybrid combinations was intermediate between the two parents. There were also differences in the size of D-type larvae at 24 hours after fertilization, and the 24-hour D-type larvae of bay scallops were larger than those of purple scallops.

Embodiment 3

[0014] Embodiment 3: after obtaining pure purple (ZZ), pure sea (HH), purple sea (ZH) and sea purple (HZ) total 4 combined populations as in embodiment 1, follow up and measure the growth situation of each group of larvae, it can be seen that The growth rate of the purple scallop was slower than that of the bay scallop (p<0.001, ANOVA), and the growth of the hybrid group was between the two female parents at the beginning, but the growth rate increased with age, showing a female parent effect and increased hybrid Advantage.

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Abstract

The invention provides a technique for preparing hermaphrodite scallop interspecific hybrid fingerlings, which relates to a marine shellfish breeding technique. Purple scallops and bay scallops are fattened and induced to be mature respectively so as to reach sexual maturity at the same time, and then are synchronously induced to lay eggs and discharge sperms respectively, so that sperms and eggs of two types of scallops are obtained respectively to perform interspecific cross insemination. The purple scallops and the bay scallops are hermaphrodite, both belong to an Argopecten category, have the same number of chromosomes, and have complementary superiority and traits. Hybrid groups are high in both fertilization rate and hatching rate and normal in larvae development. Two types of scallop parents adopted have complementary superiority and traits, thereby having significant heterosis in size, growth, adaptability and other aspects. As two types of scallops are both hermaphrodite, parent scallops are easy to purifym and the offspring with greater heterosis can be obtained by hybridizing the purified parent scallops.

Description

Technical field: [0001] The invention relates to a marine shellfish breeding technique, in particular to a technique for preparing interspecific hybrid seedlings of hermaphroditic scallops. Background technique: [0002] The scallop aquaculture industry in northern my country is currently dominated by bay fans (Argopectenirradians), and the output and aquaculture area account for more than 50% of the scallop aquaculture. The bay scallop grows fast, has a wide range of temperature adaptation (-1~31℃), and has strong stress resistance. It is very suitable for farming in the northern seas of my country. The price is lower than similar products, so the existing domestic bay scallop varieties cannot meet the needs of export. [0003] Purple scallop (Argopecten purpuratus) is a fast-growing medium-sized scallop native to the South Pacific. It can reach commercial size (9cm) after 13-18 months of growth, and the maximum shell length can reach 15cm. The purple scallop has a large s...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王春德刘保忠李继强刘升平
Owner QINGDAO AGRI UNIV
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