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Tissue culture rapid propagation method for sugarcane new Taisugar No.10

A tissue culture, Xintaitang technology, applied in the field of biotechnology engineering, can solve the problems of the rapid propagation method of tissue culture of sugarcane Xintaitang No. 10, etc., and achieve the effect of easy popularization, strong operability and high output.

Inactive Publication Date: 2012-05-30
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the rapid propagation method of sugarcane Xintaitang No. 10 tissue culture in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Axillary bud propagation and culture: select sugarcane shoots that grow robust for 6-8 months and have excellent properties as explants. Take the cane stem 40cm below the stem tip and scrub it with 70% alcohol once, then cut the cane stem below 10cm from it and soak it with 0.1% mercury + 2 drops of Tween disinfectant for 15 minutes. Rinse 3-4 times with sterile water, and then cut a 2cm-long stem section with axillary buds, and inoculate them on the axillary bud tissue culture medium (2). For the remaining part, peel off the remaining leaf sheaths to expose the buds of each stem node near the stem tip, cut off the internodes with only one axillary bud on the stem section as explants, and inoculate them into the axillary bud propagation medium, namely MS. 6-BA 1.5~3.0mg / L and NAA 0.1~0.3mg / L. After inoculation, place them at a temperature of 27±2℃, light 1500LX, and cultivate under 12 hours of light per day. After 3 to 5 days, the axillary buds will elong up to 3cm.

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PUM

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Abstract

The invention relates to a tissue culture rapid propagation method for sugarcane new Taisugar No.10. The new Taisugar No.10 has high plant, compact plant shape, straight leaf, proper width, deep green leaves in the all growth stage and high yield increase potential, belongs to a variety of early mid-maturation, high yield and sugar and wide adaptability and is suitable for planting in a main planting area of China. At present, the sugarcane adopts stem bud asexual propagation and has low propagation coefficient, and the yield and the quality of sugarcane are greatly reduced caused by the repeated invasion of various disease source substances after planting for many years; the variety degradation of a fine variety is accelerated, the economic service life of the fine variety is shortened, and the rapid popularization and application of the sugarcane variety are greatly limited. The propagation method comprises the step of carrying out the propagation of cluster buds by axillary buds in sterile sugarcane sheaths and forming a complete plant by rhizogenesis culture. The axillary bud tissue culture rapid propagation technology of the new Taisugar No.10 is suitable for the industrial rapid propagation of a sugarcane seedling.

Description

Technical field: [0001] The invention belongs to the field of biotechnology engineering, and specifically relates to the rapid propagation technology of sugarcane Xintaitang No. 10 tissue culture. Background technique: [0002] Sugarcane Xintaitang No. 10 has a tall plant, compact plant type, medium-sized stem diameter, long internodes, straight leaves, medium width, thick mesophyll, dark green leaf color throughout the growth period, fast and neat germination, strong tillering power, throughout the period The growth is vigorous and the potential for increasing production is great. It is a variety with early and middle maturity, high yield, high sugar, strong stress resistance, lodging resistance, strong ratooning, and wide adaptability. It is suitable for planting in Guangxi, Yunnan, and Guangdong, the main planting areas of China. At present, sugarcane production in the prior art uses stem buds to reproduce asexually, with a low reproduction coefficient. After years of plantin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李昆志郝永生陈丽梅
Owner KUNMING UNIV OF SCI & TECH