Method for enhancing yield of butane diacid by adding key growth factors
A technology of growth factor and succinic acid, applied in the field of fermentation engineering, can solve the problems that the species and content cannot meet the bacterial cells, the nitrogen source substitution effect is not ideal, etc., and achieve the effects of reducing the dosage, improving the utilization rate and reducing the cost.
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Embodiment 1
[0022] The present invention can use any anaerobic fermentation microbial strain producing succinic acid in the prior art to investigate the influence of key factors on the production of succinic acid. The succinic acid-producing microbial strain used in this example is: Actinobacillus succinogenes NJ113 (Actinobacillus succinogenes NJ113). This bacterium has applied for a patent and obtained authorization, and the patent authorization announcement number is CN100537744C.
[0023] First of all, the significance of various amino acids, vitamins, and metal trace elements on the growth and metabolism of the bacteria should be investigated on the synthetic medium. The specific operation is as follows:
[0024] ① Prepare the amino acid mixture. Among the 18 kinds of amino acids to be tested, one of the amino acids is removed respectively, and the other 17 kinds of amino acid mixtures without this amino acid and the mixture containing these 18 kinds of amino acids are prepared with ...
Embodiment 2
[0048] In this example, the same bacterial species, fermentation conditions and experimental method as in Example 1 are used to add the key factor glutamic acid to the hydrolyzate of waste beer yeast with cheap nitrogen source. The specific method is as follows:
[0049] The method used in this example to measure the total nitrogen content in the hydrolyzate of spent beer yeast is the Kjeldahl method.
[0050] Add 900mL of beer waste yeast hydrolyzate (total nitrogen concentration is 2.4g / L) in 3L fermentation tank, add Na 2 HPO 4 15g / L and KH 2 PO 4 4g / L, add 500mL of water, sterilize at 121°C for 15min, add 400mL of sterilized glucose with a concentration of 500g / L, 100mL of seed solution, and 100mL of 17.4g / L glutamic acid solution sterilized by filtration. At this time, the concentration of glutamic acid in the medium is shown in Table 1, the total nitrogen content is 1.1g / L, which is equivalent to the total nitrogen content of 10g / L yeast powder corresponding to the co...
Embodiment 3
[0057] In this example, for the strain Actinobacillus succinogenes 130Z (ATCC 55618), the key growth factors of the strain are determined first, and the method is the same as in Example 1.
[0058] The results showed that when glutamic acid, methionine, cysteine, and biotin were lacking in the culture medium, the fermentation effect was poor, glucose remained, and the yield of succinic acid was relatively low. The results are shown in Table 7.
[0059] Table 7 lacks the fermentation effect of growth factor
[0060]
[0061] After determining the key growth factors, add key amino acids and key vitamins to the cheap nitrogen source soybean meal hydrolyzate, the specific method is as follows:
[0062] The method for measuring the total nitrogen content in the soybean meal hydrolyzate used in this example is the same as that in Example 2.
[0063] Add soybean meal hydrolyzate 796mL (total nitrogen concentration is 2.8g / L) in 3L fermentation tank, add Na 2 HPO 4 15g / L and KH ...
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