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Method for measuring release rate of non-polar or low-polar flavor component in smoke-free tobacco product

A technology for smokeless tobacco and aroma components, applied in the field of tobacco

Active Publication Date: 2010-06-02
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, most foreign studies on the release of related components in smokeless tobacco products focus on nicotine [In Vitro Release of Nicotine From Chewing Gum Formulations, Dissolution Technologies 2004: 12-15], more flavor components in smokeless tobacco products The research on release behavior has not been reported at home and abroad

Method used

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  • Method for measuring release rate of non-polar or low-polar flavor component in smoke-free tobacco product
  • Method for measuring release rate of non-polar or low-polar flavor component in smoke-free tobacco product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Sample preparation: prepare artificial saliva and 150ppm methyl nicotinate (standard, chromatographically pure) dichloromethane solution;

[0024] 2. Weigh 0.5g of two smokeless tobacco products of equal mass and put them in a 50ml Erlenmeyer flask, add 10ml artificial saliva to one part and 10ml dichloromethane solution in the other part, and seal the Erlenmeyer flask, then Oscillate for 20 minutes at a speed of 120 rpm;

[0025] 3. Extract the saliva extracts of smokeless tobacco products with methyl nicotinate (standard) methylene chloride solution twice, 5ml each time, and combine the extracts;

[0026] 4. Add 5g of anhydrous sodium sulfate to the dichloromethane extract, refrigerate for 12h and concentrate to 1ml;

[0027] 5. Instrument analysis: Chromatographic column: DB-WAXER (30m×0.25mmi.d.×0.25μmd.f.)

[0028] Detector: NPD

[0029] Carrier gas, flow rate: N2, 1.8ml / min

[0030] Temperature of inlet and detector: 270℃

[0031] Heating program: 50℃(1min)2℃ / min→200℃;

[0...

Embodiment 2

[0039] 1. Sample preparation: prepare artificial saliva and 100ppm phenethyl acetate (standard) dichloromethane solution;

[0040] 2. Weigh two 0.9g portions of smokeless tobacco products of equal mass; put them in a 50ml Erlenmeyer flask, add 20ml artificial saliva to one portion, add 20ml dichloromethane solution to the other portion, and seal the Erlenmeyer flask, then Oscillate for 40 minutes at a speed of 200 rpm;

[0041] 3. Extract the saliva extracts of smokeless tobacco products with phenethyl acetate (standard, chromatographically pure) dichloromethane solution twice, 10ml each time, and combine the extracts;

[0042] 4. Add 10g of anhydrous sodium sulfate to the dichloromethane extract, refrigerate for 24h and concentrate to 1ml;

[0043] 5. Instrument analysis:

[0044] Chromatographic column: HP-5 capillary column (60m×0.2mm i.d.×0.33μm d.f.)

[0045] Detector: FID

[0046] Carrier gas, flow rate: N2, 0.8ml / min

[0047] Temperature of inlet and detector: 270℃

[0048] Heating ...

Embodiment 3

[0061] 1. Sample preparation: prepare artificial saliva and 50ppm phenethyl propionate (standard, chromatographically pure) dichloromethane solution;

[0062] 2. Weigh two 0.6g portions of smokeless tobacco products of equal mass and put them in a 50ml Erlenmeyer flask, add 16ml artificial saliva to one portion, and 16ml dichloromethane solution in the other portion, and seal the Erlenmeyer flask. Oscillate for 30 minutes at a speed of 150 rpm;

[0063] 3. Extract the saliva extract of smokeless tobacco products with phenethyl propionate (standard) dichloromethane solution twice, 8ml each time, and combine the extracts;

[0064] 4. Add 8g of anhydrous sodium sulfate to the dichloromethane extract, refrigerate for 18h and concentrate to 1ml;

[0065] 5. Instrument analysis: Capillary column: HP-INNOWAX 30m×0.25mmi.d×0.25μmd.f

[0066] Detector: FID

[0067] Carrier gas, flow rate: N2, 1.0ml / min

[0068] Inlet and detector temperature: 250℃, detector temperature 270℃

[0069] Heating progra...

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Abstract

The invention discloses a method for measuring the release rate of a non-polar or low-polar flavor component in a smoke-free tobacco product, which is mainly used for determining the release rate of the non-polar or low-polar flavor component in the smoke-free tobacco product by performing preliminary treatment and apparatus analysis on the smoke-free tobacco product. The method specifically comprises the following steps: weighing two parts of equivalent smoke-free tobacco products, adding artificial saliva and a methylene dichloride standard substance solution respectively, sealing and oscillating the mixture, extracting saliva extract twice with the equivalent methylene dichloride standard substance solution, combining the extract liquors, drying the combined extract liquor for cold storage and then feeding a sample in a concentration way; performing separation and detection on an extracted component with GC and GC-MS, retrieving and determining the content of the non-polar or low-polar flavor component according to a retaining time and a mass spectrum database, and comparing the content of the extracted component in the saliva with the content of the directly extracted component of the methylene dichloride solution so as to obtain the release rate of the flavor extracted component of the smoke-free tobacco product. The method has the advantages of simple process, high analysis speed, good accuracy, unlimited sample physical state and the like.

Description

Technical field [0001] The invention belongs to the field of tobacco, and specifically is a method for measuring the release rate of non-polar or weakly polar flavor components in smokeless tobacco products. Background technique [0002] At present, cigarette consumption is the main form of tobacco consumption in my country. As the public pays more and more attention to health, social restrictions on smoking in public places are becoming stricter. As one of the member states of the World Health Organization, my country has signed the "Framework Convention on Tobacco Control." [0003] Although smoking has an impact on health, due to the unique charm of tobacco, the total number of tobacco consumers in the world is still on the rise. Due to health and social pressure, the tobacco industry is striving to explore a form of tobacco consumption that can not only meet the needs of consumers but also reduce the harm to the health of smoking. The hazards of smoking mainly come from the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N1/28
Inventor 宋瑜冰宗永立马骥李鹏张杰彭书海
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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