Human epididymal expression sperm binding protein HEL-44 as well as encoding gene and application thereof

A HEL-44 and protein-binding technology, applied in the fields of biotechnology and medicine, can solve the problems of poorly understood protein functions and achieve the effect of less side effects
CN101724040AInactive Publication Date: 2010-06-09李建远
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
李建远
Publication Date
2010-06-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-44 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-44 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located in the equatorial region of a mature sperm, an RT-PCR result indicates that a gene has expression in various tissues such as an epididymis, a testis, a heart, a lung, a liver, a spleen, a kidney, a stomach and the like, and a band of 23 KD is detected in the body part of the epididymis by using Western blot. The HEL-44 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-44 protein can be widely used for the research field of reproductive medicine.
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Description

technical field

[0001] The invention belongs to the field of biotechnology and medicine. Specifically, the present invention relates to a new human epididymis expressing sperm-binding protein HEL-44 and its coding gene and application Background technique

[0002] The development of human beings in this century is facing two serious problems: on the one hand, the global population is rapidly expanding and is expected to reach 9 billion in 2050; on the other hand, according to the World Health Organization survey, about 15% of couples are infertile The problem is that the incidence of infertility in developed countries has soared in the past 10 years, and it can be as high as 30% in developed countries in Europe, of which male factors account for about half. The World Health Organization predicts that with the increase of environmental pollution and sexually transmitted diseases and other pathogenic factors, infertility will become the third major disease after tumors and ca...

Claims

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