Anti-wrinkle composition for external applications to the skin containing biflavonoid derivatives

A technology of flavonoids and derivatives, which is applied in the field of anti-wrinkle compositions for skin external use containing bisflavonoid derivatives, and can solve problems such as instability, skin irritation stability, and skin irritation

Inactive Publication Date: 2010-06-09
AMOREPACIFIC CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although retinoids have positive effects such as improving wrinkles or elasticity, they have problems in that they irritate the skin even when applied in a small amount, and are rather unstable so that they are easily oxidized and denatured when exposed to the air, which Problems have caused limitations in its application
Therefore, research to make retinoids more stable has been going on, but the problem of skin irritation and stability has not been solved

Method used

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  • Anti-wrinkle composition for external applications to the skin containing biflavonoid derivatives
  • Anti-wrinkle composition for external applications to the skin containing biflavonoid derivatives
  • Anti-wrinkle composition for external applications to the skin containing biflavonoid derivatives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: the extraction of sumephedrone

[0060] 1.8 kg of dried Selaginella was cut into small pieces, and then extracted using methanol at 80° C. for 4 hours, and the extraction was repeated three times. The obtained methanol extract was concentrated under reduced pressure. 78.7 g of the concentrate was dissolved in water added with dichloromethane (as an extraction solvent), followed by extraction three times. A dichloromethane-soluble fraction (21.7 g) was obtained, and the residue was extracted three more times using ethyl acetate or butanol as an extraction solvent. As a result, ethyl acetate-soluble components (6.8 g), butanol-soluble components (7.8 g) and residual water components (16.2 g) were obtained.

[0061] Silica gel column chromatography was performed on 6.8 g of ethyl acetate-soluble fraction. A mixed solvent containing chloroform, methanol, and water in a ratio of 10:1:0.1 (vol / vol) was used as a column elution buffer. Column chromatography w...

Embodiment 2

[0063] Embodiment 2: Determination of the structure of Panduratin A compound

[0064] In order to determine the structure of the single material separated in Example 1, measured at 500MHz and 125MHz (solvent: DMSO) respectively 1 H-NMR spectrum and 13 C-NMR spectrum. The result is as follows.

[0065] Will 1 H-NMR and 13 The results of C-NMR were compared with those of traditional studies to identify the structure, and the references are listed below.

[0066] Lin, L.-C., Kuo, Y.-C., and Chou, C.-J. (2000) Cytotoxic biflavonoids from Selaginella delicatula. J. Nat. Prod., 63, 627-630.

[0067] Markham, K.R., Sheppard, C., and Geiger, H. (1987) 13 C NMR studies of some naturally occurring amentoflavone and hinokiflavone biflavonoids. Phytochemistry, 26, 3335-3337.

[0068] Chen, S.-C., Liu, H.-K., and Kuo, Y.-H. (2004) Two new compounds from the leaves of Calocedrus microlepic var. formosana. Chem. Pharm. Bull., 52, 762- 763.

[0069] Chari, V.M., Ilyas, M., Wagner, H....

experiment example 1

[0092] Experimental Example 1: Biosynthesis of Procollagen

[0093] Fibroblasts isolated from human skin were seeded in 24-well plates (10 6 cells / well), followed by culturing until they grow 90%. Then, these cells were cultured in serum-free medium for 24 hours. The 4-substituted benzoic acid derivative compound having 3,4-methylenedioxybenzene or 3,4-ethylenedioxybenzene moiety obtained in Examples 1 to 2 was dissolved in a serum-free medium, and the 10 -4 The concentration of the medium was treated, followed by CO 2 Incubate for 24 hours in the incubator. The supernatant was separated and procollagen levels were determined using a type 1 procollagen ELISA kit. The results are shown in Table 1, and the biosynthetic ability was expressed by the ratio to the untreated group (considered 100%).

[0094] [Table 1]

[0095] compound

[0096] As shown in Table 1, it was confirmed that the biflavonoid derivatives can promote collagen biosynthesis.

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Abstract

The present invention relates to a cosmetic composition for improving skin wrinkles, which contains biflavonoid derivatives as an active ingredient. The cosmetic composition containing the biflavonoid derivatives of the present invention not only is safe for application on the skin without side-effects but also improves wrinkles caused by UV through procollagen production and MMP-I inhibition.

Description

technical field [0001] The present invention relates to a cosmetic composition containing a biflavonoid derivative as an active ingredient for improving skin wrinkles caused by aging. Background technique [0002] The present invention relates to an anti-wrinkle cosmetic composition containing a biflavonoid derivative as an active ingredient, which can be safely applied to the skin without side effects, and which can induce procollagen production, inhibit MMP-1, and have the effect of improving ultraviolet ray The effect of causing wrinkles. [0003] Every living body experiences aging as it ages, and skin is no exception. People are always trying to delay aging and continue to explore questions about "what is aging" and "what causes aging". [0004] Skin aging can be mainly divided into two types according to the causes; one is intrinsic aging caused by the aging of the structure and physiological functions of the skin, and the other is extrinsic aging caused by cumulativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/49A61K8/97A61Q19/08
CPCA61Q19/08A61Q17/04A61K8/498A61K8/97A61K8/9739A61K8/9771A61K8/9789A61K8/9794A61K8/9749
Inventor 李灿雨禹银兰金汉星李松伊崔贤贞金志映金真雄金德姬金汉坤张利燮
Owner AMOREPACIFIC CORP
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