Donor heart preserving solution with donor heart preservation delaying effect and method for preparing same

Abstract

The invention discloses donor heart preserving solution with donor heart preservation delaying effect and a method for preparing the same. The donor heart preserving solution with donor heart preservation delaying effect comprises 4.68 g of sodium chloride, 1.86 g of potassium chloride, 0.57 g of magnesium chloride, 0.01 g of calcium chloride, 2.18 g of glucose, 0.91 g of mannitol, 10 Mug of insulin, 10 g of PEG-20, 50 mg of furosemide, 5 Mug of PGE1, 11.52 g of histidine and the balance of double distilled water. The method for preparing the donor heart preserving solution with donor heart preservation delaying effect comprises the following steps: adding 800 ml of double distilled water to a container of more than 1000 ml; adding 4.68 g of sodium chloride, 1.86 g of potassium chloride, 0.57 g of magnesium chloride and 0.01 g of calcium chloride to the container in sequence, and stirring the solution in the container until the color of the solution being clear; adding 11.52 g of histidine, 2.18 g of glucose, 0.91 g of mannitol and 10 g of PEG-20 to the container, and stirring the solution in the container until the color of the solution being clear; adding 10 Mug of insulin, 50 mg of furosemide and 5 Mug of PGE1 to the container, and stirring the solution in the container until the color of the solution being clear; adjusting the pH value of the solution to 7.65, the osmotic pressure to 310-315 mOsm / L and the constant volume of the double distilled water to 1000 ml; and standing the solution at the low temperature of 4 DEG C for preservation. The invention has the advantages that the donor heart can be preserved for a longer time of 10h, the calcium ions of trace amount contained in the donor heart preserving solution can avoid the calcium paradoxical phenomenon, and the donor heart preserving solution can prevent the intracellular acidosis, promote the energy regeneration and prevent the cardiac vasospasm at low temperature.

Description

【Technical field】 [0001] The invention relates to the field of organ preservation solutions, in particular to a donor heart preservation solution with a delayed preservation effect and a preparation method thereof. 【Background technique】 [0002] The protective effect of heart transplantation donor heart directly affects the survival rate of transplantation, and also has an important impact on the long-term effect after transplantation. At present, cryopreservation after cardiac arrest and perfusion is still the most important clinical application method. But the effective storage time is less than 4 to 6 hours. Exploring an effective preservation solution is the key to improving the effect of simple low temperature preservation. [0003] The purpose of myocardial preservation solution in heart transplantation is to prevent cell swelling under low temperature conditions, prevent intracellular acidosis, reduce interstitial water, reduce the generation and damage of oxygen f...

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