Bioreactor for culturing tissue engineered esophageal stent

Abstract

The invention discloses a bioreactor for culturing a tissue engineered esophageal stent, which is characterized by comprising a box, a transmission connecting plate, a transmission shaft and a driven shaft. The transmission shaft and the driven shaft are arranged coaxially, wherein the transmission shaft is coupled with the box in a sealing way, a shaft seat is arranged in the box, the driven shaft is coupled with the shaft seat, the transmission shaft is fixedly provided with a first stent connector, the driven shaft is fixedly provided with a second stent connector, one end of the transmission connecting plate is fixedly arranged on the first stent connector, the other end of the transmission connecting plate is fixedly arranged on the second stent connector, and a rotating drive deviceis arranged on the transmission shaft. The invention has the advantages that: inoculation can be evened on the esophageal epithelial cell on the cavity surface of the esophageal stent, and during theculture of the esophageal stent, the box of the reactor can not move, while the esophageal stent can rotate, so that the culture solution in the box can exert obvious forces such as shear stress and the like on cells on the esophageal stent and the esophageal stent constructed in vitro can simulate the working environment of the normal esophagus to perform the functions of the esophagus.

Description

technical field [0001] The invention relates to a device for constructing and cultivating tissue engineered organs, in particular to a bioreactor for cultivating tissue engineered esophageal scaffolds. Background technique [0002] From the perspective of histological structure, the human esophagus is a hollow tube about 25 centimeters long and 1.9 centimeters wide. It is located behind the throat and chest cavity, connects the throat and stomach, and mainly plays the role of transporting food and water. From the perspective of microscopic structure, the esophagus is composed of mucosa, submucosa, muscular layer and adventitia. Composed of non-keratinized stratified squamous epithelial cells, epithelial cells grow on the basement membrane, which provides special proteins, growth factors and other nutrients required for epithelial cell growth to regulate the proliferation, differentiation, and migration of epithelial cells The epithelial cells and the basement membrane toget...

AI Technical Summary

Problems solved by technology

Studies have shown that dynamic bioreactors are superior to static incubators in many aspects of cell culture. It can achieve uniform and high-density seeding of cells on scaffolds constructed in vitro. An important tool for construction, the current dynamic bioreactor basically rotates the entire reactor, so that the bracket installed in the reactor can rotate together, so as to achieve the purpose of uniformly seeding the cells on the surface of the inner cavity of the bracket, but this reaction When the reactor is in use, it must first be sealed, and the reactor must be filled with culture solution, so as to avoid the influence of the cultivation effect due to the large number of air bubbles in the reactor due to rotation; When one of them rotates together, the shear stress given by the culture medium to the scaffold or the cells seeded on the scaffold is actually not obvious, or even non-existent, so that the cell-scaffold complex cannot be given a growth environment similar to that in the human body and its function will be affected

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