Culture apparatus and applications thereof in cell culture

A culture device and cell technology, applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve the problem of low oxygen dissolution efficiency, lack of cell culture attachment and growth, and expansion of unfavorable culture volume Process control and other issues to achieve the effect of avoiding pollution and improving work efficiency

Inactive Publication Date: 2010-06-23
HANGZHOU AMPROTEIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing cell culture equipment has low oxygen dissolution efficiency and lacks the carrier required for cell culture attachment and growth. Cells can only be suspended in the cell culture medium or adhered to the surface of the fixture in the culture equipment for growth, which is not conducive to the realization of the culture capacity. Expansion and control of the cultivation process

Method used

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  • Culture apparatus and applications thereof in cell culture
  • Culture apparatus and applications thereof in cell culture
  • Culture apparatus and applications thereof in cell culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, as figure 1 As shown: the transmission device 2 enables the carrier module 3 to gradually change from fully exposed to the gas above the liquid surface to fully immersed in the culture solution in the airtight container 1, and then gradually change from fully immersed in the culture solution to fully exposed to the gas In this way, it moves back and forth again and again, and the movement method is up and down piston movement.

Embodiment 2

[0037] Embodiment 2, as figure 2 As shown: the transmission device 2 enables the carrier module 3 to gradually change from fully exposed to the gas above the liquid surface to fully immersed in the culture solution in the airtight container 1, and then gradually change from fully immersed in the culture solution to fully exposed to the gas In this way, it moves back and forth again and again, and the movement method can be a pendulum movement.

Embodiment 3

[0038] Embodiment 3, as indicated image 3 As shown, a plurality of carrier modules 3 are distributed on the transmission device at a certain distance. When the transmission device 1 drives the carrier module 3 to move in the airtight container 1, part of the carrier module is exposed to the air, while the other part is immersed in the culture solution 4. With the movement of the transmission device, the exposed carrier part is gradually immersed in the In the culture solution, the other part submerged in the culture solution is gradually exposed to the air, so that the carrier module moves periodically in the gas or in the culture solution. The airtight container 1 is provided with a liquid inlet 5 and a liquid outlet 6 .

[0039] After a large number of experiments, it has been proved that the maximum cell number and state of the cells harvested by this equipment can meet the actual needs.

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PUM

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Abstract

The invention relates to a culture apparatus which at least comprises a closed container, a transmission gear and a carrier module. When the culture apparatus is driven by the transmission gear, the carrier module in the closed container generates a continuous movement, so that the carrier module is periodically exposed to the gas cavity or immersed in the culture fluid in the closed container. The device of the invention has the following advantages: 1. the carrier used by the apparatus is made of polyester fiber material, the fiber structure can provide a larger specific area for the adsorption growth of cells, and the carrier provides the cells with the best growing space; 2. after cell inoculation, since the carrier has large bearing capacity, the carrier can provide sufficient space for cell amplification without other seed chain amplification operations, thereby avoiding pollution to the maximum; 3. the carrier module of the apparatus is a disposable part, and does not need to be cleaned, disinfected and the like; and 4. the apparatus can be installed in a 100-stage self cleaning system, and thus, inoculation, culture and harvesting of cells can be operated in a safe and clean environment.

Description

technical field [0001] The invention relates to a device for culturing cells and its application in cell culture. Background technique [0002] During cell culture, a large amount of oxygen and a relatively static environment are required. However, the existing cell culture equipment has low oxygen dissolution efficiency and lacks the carrier required for cell culture attachment and growth. Cells can only be suspended in the cell culture medium or adhered to the surface of the fixture in the culture equipment for growth, which is not conducive to the realization of the culture capacity. The expansion and control of the cultivation process. Contents of the invention [0003] In order to overcome the above defects, the object of the present invention is to provide a culture device and its application in cell culture, the device can perform high-density, long-term continuous culture of cells. [0004] In order to achieve the above object, the present invention adopts the fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 徐江运惠觅宙原世平谢玉萍李峦峰陶苒胡福林
Owner HANGZHOU AMPROTEIN BIOTECH
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