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Actinopolyspora erythraea

A technology of actinomycetes and erythromycin, which is applied in the field of microbiology and can solve the problems of reduced curative effect of erythromycin drugs

Inactive Publication Date: 2010-06-23
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, with the continuous emergence of clinical drug-resistant bacteria and the enhancement of drug resistance of these bacteria, the efficacy of clinically used erythromycin drugs is gradually decreasing, and further development of new antibiotic drugs is required

Method used

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  • Actinopolyspora erythraea

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Embodiment

[0022] Use the erythromycin-producing polyspore actinomycete Actinopolyspora erythraea YIM 90600 of the present invention T Preparation of new compound 6,18-epoxy-14hydroxyerythromycin lactone B.

[0023] 1. Actinopolyspora erythraea YIM 90600 T Fermentation treatment of strains:

[0024] a. Cultivate ISP 4+10% NaCl on a slant, and cultivate at 37°C for 15 days until the growth of aerial spores;

[0025] b. Seed culture ISP 4 (no agar) + 10% NaCl, 37 ° C, 250 rpm, culture for 10 days;

[0026] c. Fermentation culture In the fermentation medium, at 37 degrees Celsius, 250 rpm, cultivated for 30 days, the composition and proportion of the fermentation medium: soybean powder 20g, peptone 2g, glucose 20g, starch 5g, yeast extract 2g, NaCl 74g , K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, Na 2 SO 4 10H 2 O 25g, KCl 15g, MgCl 2 ·6H 2 O 10g, CaSO 4 2H 2 O 5g, CaCO 3 2g, 1000ml deionized water, pH 7.8;

[0027] Operation process: Take a spore of the strain that has grown in...

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Abstract

The invention relates to the technical field of microbes, in particular to a microbe bacterial strain in which a new compound can be obtained by separating in the fermentation liquor. The invention is named as Actinopolyspora erythraea YIM 90600T, and is preserved in a depositary institution appointed by the State Intellectual Property OfficeState Intellectual Property Office with the preserving number of CCTCC M208243. The Actinopolyspora erythraea of the invention is separated from saline soil in AydingkolHu in Xinjiang of China. The depositary institution appointed by the State Intellectual Property OfficeState Intellectual Property Office is the China Center for Type Culture Collection in Wuhan University in China. The postcode is 430072, and the preserving data is Oct. 9, 2007. A new erythrocin analogue having antibacterial tumour cytoactive (MTT method) can be obtained by separating in the fermentation liquor of the Actinopolyspora erythraea of the invention can separate.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a microbial strain capable of separating and obtaining new compounds in its fermented liquid. Background technique [0002] There are many kinds of derivatives of erythromycin, and erythromycin C is used for analysis. In its 14-membered macrocyclic structure, there is no hydroxyl group at the 14th position, and no three-membered ring is formed between the 6th and 18th positions. The synthesis and development of erythromycin drugs mainly focus on the substitution of active hydroxyl groups such as 3, 5, 6, 11 and 12 on the 14-membered ring and the resubstitution reaction of substituents, and a good erythromycin Clinical drug. However, with the continuous emergence of clinical drug-resistant bacteria and the enhancement of drug resistance of these bacteria, the efficacy of clinically used erythromycin drugs is gradually decreasing, and further development of new antibiotic drugs i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/62C12R1/01
Inventor 唐蜀昆李文均赵立兴徐丽华姜成林
Owner YUNNAN UNIV
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