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Application of bacillus licheniformis alkali protease as asymmetrical direct Aldol reaction catalyst

A technology of Bacillus licheniformis and protease, which is applied in the direction of fermentation to achieve the effects of environmental friendliness, good application prospects and high selectivity

Inactive Publication Date: 2010-06-23
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the aldol reaction catalyzed by bacillus licheniformis alkaline protease (BLAP), especially the asymmetric direct aldol reaction, has not been reported yet.

Method used

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  • Application of bacillus licheniformis alkali protease as asymmetrical direct Aldol reaction catalyst
  • Application of bacillus licheniformis alkali protease as asymmetrical direct Aldol reaction catalyst
  • Application of bacillus licheniformis alkali protease as asymmetrical direct Aldol reaction catalyst

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Embodiment Construction

[0018] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

[0019] The Bacillus licheniformis alkaline protease (EC 3.4.21.14) used in the preferred embodiment is derived from Bacillus licheniformis 2709, purchased from Wuxi Xuemei Enzyme Technology Co., Ltd., with an enzyme activity of 200 U / mg.

[0020] 1. Condition optimization of the asymmetric direct Aldol reaction catalyzed by Bacillus licheniformis alkaline protease

[0021] (1) Optimization of organic solvents

[0022] The present invention takes the reaction of acetone and p-cyanobenzaldehyde as a model reaction, and investigates the organic solvent of the asymmetric direct Aldol reaction catalyzed by bacillus licheniformis alkaline protease.

[0023] The method is as follows: add bacillus licheniformis alkaline protease (200mg), organic solvent (5ml), p-cyanobenzaldehyde (200mg, 1.5mmol) and deionized water (0.75ml) in the round bottom...

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Abstract

The invention discloses application of bacillus licheniformis alkali protease as an asymmetrical direct Aldol reaction catalyst, which has the characteristics of high efficiency, high selectivity, recycle, economy, environment protection and the like and has good application prospects.

Description

technical field [0001] The invention relates to a new application of the bacillus licheniformis alkaline protease, in particular to the application of the bacillus licheniformis alkaline protease as an asymmetric direct Aldol reaction catalyst. Background technique [0002] Stereoselective formation of C–C bonds is an important aspect of synthetic organic chemistry. The aldol condensation reaction (aldol reaction) is one of the most efficient C–C bond formation reactions. In the past, most asymmetric aldol reactions were carried out with modified substrates, that is, derivatized substrate ketones into enols, which can activate substrate ketones on the one hand and effectively inhibit the occurrence of intermolecular interactions between the same species on the other hand. reaction. Although many chiral catalysts have achieved good asymmetric catalytic effects in this regard, this type of reaction is not as good as the direct aldol reaction between unmodified ketones and al...

Claims

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Application Information

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IPC IPC(8): C12P7/26
Inventor 何延红官智李海虹
Owner SOUTHWEST UNIVERSITY
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