Biodegradation test method of synthetic oil and vegetable oil-based lubricating oil product
A biodegradable, vegetable oil-based technology, applied in the field of biodegradation testing of synthetic oils and vegetable oil-based lubricating oil products, achieves the effects of low cost, stable data reproducibility and repeatability, and wide application prospects
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[0029] 1. Culture medium preparation
[0030] Acclimatization medium: KH 2 PO 4 3.4g; Na 2 HPO 4 1.5g; (NH 4 ) 2 SO 4 4.0g; MgSO 4 0.7g; yeast powder 0.01g; castor-based lubricating oil 2g; tap water 1000mL; adjust the pH to 7.2-7.6
[0031] Seed medium: peptone 1.4%, NaCl 0.6%, glucose 0.3%, beef extract 0.5%, KH 2 PO 4 0.5%, K 2 HPO 4 0.4%; the culture temperature is 37°C, and the pH value of the medium is 7.5.
[0032] Degradation medium mineral nutrient solution: KH 2 PO 4 3.4g; Na 2 HPO 41.5g; (NH 4 ) 2 SO 4 4.0g; MgSO 4 0.7g; yeast powder 0.01g; tap water 1000mL; adjust the pH to 7.2-7.6.
[0033] 2. Soil collection and domestication of degrading bacteria
[0034] 1) Requirements for collecting soil: collect the activated sludge from the aeration tank, and the sampling depth is 15-30cm; the collection equipment is a sterilized soil collector and a plastic sealing bag.
[0035] 2) Soil and treatment: After the soil is collected and aerated f...
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