Biodegradation test method of synthetic oil and vegetable oil-based lubricating oil product

A biodegradable, vegetable oil-based technology, applied in the field of biodegradation testing of synthetic oils and vegetable oil-based lubricating oil products, achieves the effects of low cost, stable data reproducibility and repeatability, and wide application prospects

Inactive Publication Date: 2010-06-23
TIANJIN NANKAI UNIV CASTOR ENG SCI & TECH
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  • Biodegradation test method of synthetic oil and vegetable oil-based lubricating oil product
  • Biodegradation test method of synthetic oil and vegetable oil-based lubricating oil product
  • Biodegradation test method of synthetic oil and vegetable oil-based lubricating oil product

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Embodiment Construction

[0029] 1. Culture medium preparation

[0030] Acclimatization medium: KH 2 PO 4 3.4g; Na 2 HPO 4 1.5g; (NH 4 ) 2 SO 4 4.0g; MgSO 4 0.7g; yeast powder 0.01g; castor-based lubricating oil 2g; tap water 1000mL; adjust the pH to 7.2-7.6

[0031] Seed medium: peptone 1.4%, NaCl 0.6%, glucose 0.3%, beef extract 0.5%, KH 2 PO 4 0.5%, K 2 HPO 4 0.4%; the culture temperature is 37°C, and the pH value of the medium is 7.5.

[0032] Degradation medium mineral nutrient solution: KH 2 PO 4 3.4g; Na 2 HPO 41.5g; (NH 4 ) 2 SO 4 4.0g; MgSO 4 0.7g; yeast powder 0.01g; tap water 1000mL; adjust the pH to 7.2-7.6.

[0033] 2. Soil collection and domestication of degrading bacteria

[0034] 1) Requirements for collecting soil: collect the activated sludge from the aeration tank, and the sampling depth is 15-30cm; the collection equipment is a sterilized soil collector and a plastic sealing bag.

[0035] 2) Soil and treatment: After the soil is collected and aerated f...

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Abstract

The invention relates to a biodegradation test method of a synthetic oil and vegetable oil-based lubricating oil product. The collected activated sludge is accessed to a synthetic oil and vegetable oil-based lubricating oil degradation culture medium after acclimatization and the tested microorganism bacteria solution biomass in the degradation culture medium and the lubricating oil organic matter residue in a liquid phase culture medium are served as an evaluation method and standard for evaluating the biodegradation rate of the synthetic oil and vegetable oil-based lubricating oil after seven days of continuous shaking culture under constant temperature. The biodegradation test method is a biodegradation evaluation method of the synthetic oil and vegetable oil-based lubricating oil, which has simple and convenient operation, good data reproducibility and stable repeatability and is applicable to biodegradable lubricating oil with different specifications and viscosity grades. The method is applied in the biodegradation tests of castor-based fully-synthetic high-performance lubricating oil products and uses the castor oil which has the degradation rate of about 96% in the literature reports as the reference oil, the tested degradation rate of the castor oil is 97%, and the degradation rate of the castor-based fully-synthetic high-performance lubricating oil reaches up to 85%.

Description

【Technical field】 [0001] The invention relates to an evaluation method of biodegradable lubricating oil, in particular to a biodegradation testing method of synthetic oil and vegetable oil-based lubricating oil products. 【Background technique】 [0002] At present, many countries in the world have started the development and use of biodegradable lubricating oil, so at the same time there will be the establishment of corresponding biodegradable performance standards. The standards of lubricating oil biodegradability in each country must be determined according to the social status of each country. Certainly. The biodegradation methods of lubricating oil products mainly include: one is to evaluate the biodegradation rate by measuring the consumption of oxygen and the generation of carbon dioxide; the other is to evaluate the biodegradation rate by measuring the residual amount of lubricating oil after microbial cultivation. These two methods are currently the most widely used ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02G01N21/31G01N21/84
CPCC12P1/00C02F1/00C02F9/00G01N33/56911
Inventor 吴新世张盈孟祥云
Owner TIANJIN NANKAI UNIV CASTOR ENG SCI & TECH
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