Method for detecting early apoptosis with electrorotation technology

Abstract

The invention provides a method for detecting early apoptosis with an electrorotation technology, which comprises the steps: (1) for cell to be detected, adding apoptosis inducing factor in the liquid medium of the cell to make the cell have apoptosis, (2) making the cell to be detected suspend on the liquid medium, and adding the liquid medium into an electrorotation detection cell, (3) adoptingliquid medium which is the same as the liquid medium in the electrorotation detection cell, and perfusing the liquid medium in the electrorotation detection cell, (4) generating uniform electric field in the electrorotation detection cell to lead the cell to be detected to have electrorotation action, and (5) changing the frequency of the rotating electric field to detect the rotating speed of the cell to be detected under the corresponding condition, and obtaining an electrorotation spectrum. The invention adopts the electrorotation technology as a non-invasive method to detect the early apoptosis, ensures the accuracy of the detection, and in addition, can lead the object to be detected to keep activity, and can realize the continuous study of one batch or single body.

Description

technical field [0001] The invention relates to a method for detecting physiological changes in the apoptosis process of cells by using electrorotation technology, especially a method for detecting early apoptosis changes in cells. Background technique [0002] Apoptosis refers to the autonomous and orderly death of cells controlled by genes in order to maintain a stable internal environment. It can promptly and effectively remove excessive or potentially dangerous cells in the body, and is used to maintain the balance of various types of cells in the body. This balance is critical for promoting organ formation and maintaining the stability of the body's internal environment. The significance of apoptosis research is that it opens a new window for our cancer drug research. However, due to defects in the apoptosis control program of cancer cells and the development of drug resistance in cancer cells, cancer patients still relapse after chemotherapy in clinical practice. Cu...

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Problems solved by technology

[0011] The invention provides a method for detecting early apoptosis of cells by using electrorotation technology to solve the problem of detecting cell apoptosis in the prior art due to the different expression of receptors or proteins of different research objects, or the time of different biochemical events or activities. The points are often different, resulting in inaccurate detection of the existing technology; and the existing detection methods are invasive to the measured object, and it is impossible to continue to observe the changes of the measured object in the subsequent process

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